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Protein\u2013nanoparticle conjugation can be used in order to promote specific interactions between living systems and nanocarriers. Non\u2010covalent conjugates are less stable and more susceptible to desorption in biological media, which makes the development of engineered nanoparticle surfaces by covalent attachment an interesting topic. In this work, the surface of poly(globalide\u2010co\u2010\u03b5\u2010caprolactone) (PGlCL) nanoparticles containing double bonds in the main polymer chain is covalently functionalized with bovine serum albumin (BSA) by thiol\u2010ene chemistry, producing conjugates which are resistant to dissociation. The successful formation of the covalent conjugates is confirmed by flow cytometry (FC) and fluorescence correlation spectroscopy (FCS). Transmission electron microscopy (TEM) allows the visualization of the conjugate formation, and the presence of a protein layer surrounding the NPs can be observed. After conjugation with BSA, NPs present reduced cell uptake by HeLa and macrophage RAW264.7 cells, in comparison to uncoated NP. These results demonstrate that it is possible to produce stable conjugates by covalently binding BSA to PGlCL NP through thiol\u2010ene reaction.<\/jats:p>","DOI":"10.1002\/mabi.201900145","type":"journal-article","created":{"date-parts":[[2019,9,6]],"date-time":"2019-09-06T13:15:45Z","timestamp":1567775745000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Covalently Binding of Bovine Serum Albumin to Unsaturated Poly(Globalide\u2010Co\u2010\u03b5\u2010Caprolactone) Nanoparticles by Thiol\u2010Ene Reactions"],"prefix":"10.1002","volume":"19","author":[{"given":"Camila","family":"Guindani","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering and Food Engineering \u2013 Federal University of Santa Catarina \u2013 EQA\/UFSC \u2013 C.P. 476  88040\u2013900 Florian\u00f3polis SC Brazil"},{"name":"Max Planck Institute for Polymer Research  Ackermannweg 10 55128 Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Luise","family":"Frey","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Polymer Research  Ackermannweg 10 55128 Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Simon","sequence":"additional","affiliation":[{"name":"Department of Dermatology \u2013 University Medical Center \u2013 Johannes Gutenberg\u2010University Mainz  Langenbeckstrasse 1 55131 Mainz Germany"},{"name":"Max Planck Institute for Polymer Research  Ackermannweg 10 55128 Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaloian","family":"Koynov","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Polymer Research  Ackermannweg 10 55128 Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Schultze","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Polymer Research  Ackermannweg 10 55128 Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra R. 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