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The incorporation of porphyrins into natural or synthetic nano\u2010assemblies such as polymersomes is a strategy to improve and prolong their therapeutic capacities and to overcome their limitations as therapeutic and diagnostic agents. Here, 5,10,15,20\u2010tetrakis(1\u2010(6\u2010ethoxy\u20106\u2010oxohexyl)\u20104\u2010pyridin\u20101\u2010io)\u201021H,23H\u2010porphyrin tetrabromide porphyrin is inserted into polymersomes in order to demonstrate that the encapsulation enhances its ability to generate highly reactive singlet oxygen (<jats:sup>1<\/jats:sup>O<jats:sub>2<\/jats:sub>) upon irradiation in vitro. The photoactivation of the free and polymersome\u2010encapsulated porphyrin is evaluated by electron spin resonance and cell viability assays on three different mammalian cell lines. The results indicate that by encapsulating the porphyrin, a controlled ROS delivery within the cells is achieved, at the same time avoiding side effects such as dark toxicity, non\u2010specific porphyrin release and over time decreased activity in vitro. This work focuses on showing a not\u2010toxic model system for modern therapeutic nanomedicine, which works under mild irradiation and dosage conditions.<\/jats:p>","DOI":"10.1002\/mabi.201900291","type":"journal-article","created":{"date-parts":[[2019,12,11]],"date-time":"2019-12-11T16:45:08Z","timestamp":1576082708000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Porphyrin Containing Polymersomes with Enhanced ROS Generation Efficiency: In Vitro Evaluation"],"prefix":"10.1002","volume":"20","author":[{"given":"Myrto","family":"Kyropoulou","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Basel  Mattenstrasse 24a 4058 Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"DiLeone","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Basel  Mattenstrasse 24a 4058 Basel 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