{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:08:25Z","timestamp":1790399305756,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,2,17]],"date-time":"2020-02-17T00:00:00Z","timestamp":1581897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01NS092847"],"award-info":[{"award-number":["R01NS092847"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P41EB002520"],"award-info":[{"award-number":["P41EB002520"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIHS10 OD021624"],"award-info":[{"award-number":["NIHS10 OD021624"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The prevalence of dementia and other neurodegenerative diseases continues to rise as age demographics in the population shift, inspiring the development of long\u2010term tissue culture systems with which to study chronic brain disease. Here, it is investigated whether a 3D bioengineered neural tissue model derived from human induced pluripotent stem cells (hiPSCs) can remain stable and functional for multiple years in culture. Silk\u2010based scaffolds are seeded with neurons and glial cells derived from hiPSCs supplied by human donors who are either healthy or have been diagnosed with Alzheimer's disease. Cell retention and markers of stress remain stable for over 2 years. Diseased samples display decreased spontaneous electrical activity and a subset displays sporadic\u2010like indicators of increased pathological \u03b2\u2010amyloid and tau markers characteristic of Alzheimer's disease with concomitant increases in oxidative stress. It can be concluded that the long\u2010term stability of the platform is suited to study chronic brain disease including neurodegeneration.<\/jats:p>","DOI":"10.1002\/mabi.202000004","type":"journal-article","created":{"date-parts":[[2020,2,17]],"date-time":"2020-02-17T14:36:11Z","timestamp":1581950171000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["A Long\u2010Living Bioengineered Neural Tissue Platform to Study Neurodegeneration"],"prefix":"10.1002","volume":"20","author":[{"given":"Nicolas","family":"Rouleau","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering School of Engineering Tufts University Science &amp; Technology Center  4 Colby Street Medford MA 02155 USA"},{"name":"Initiative for Neural Science, Disease &amp; Engineering Tufts University Science &amp; Engineering Complex  200 College Avenue Medford MA 02155 USA"},{"name":"The Allen Discovery Center at Tufts University Biology Department Tufts University  200 Boston Ave. Suite 4600 Medford MA 02155 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William L.","family":"Cantley","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering School of Engineering Tufts University Science &amp; Technology Center  4 Colby Street Medford MA 02155 USA"},{"name":"Initiative for Neural Science, Disease &amp; Engineering Tufts University Science &amp; Engineering Complex  200 College Avenue Medford MA 02155 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volha","family":"Liaudanskaya","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering School of Engineering Tufts University Science &amp; Technology Center  4 Colby Street Medford MA 02155 USA"},{"name":"Initiative for Neural Science, Disease &amp; Engineering Tufts University Science &amp; Engineering Complex  200 College Avenue Medford MA 02155 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Berk","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering School of Engineering Tufts University Science &amp; Technology Center  4 Colby Street Medford MA 02155 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuang","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering School of Engineering Tufts University Science &amp; Technology Center  4 Colby Street Medford MA 02155 USA"},{"name":"Initiative for Neural Science, Disease &amp; Engineering Tufts University Science &amp; Engineering Complex  200 College Avenue Medford MA 02155 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Rusk","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering School of Engineering Tufts University Science &amp; Technology Center  4 Colby Street Medford MA 02155 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Peirent","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering School of Engineering Tufts University Science &amp; Technology Center  4 Colby Street Medford MA 02155 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cole","family":"Koester","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering School of Engineering Tufts University Science &amp; Technology Center  4 Colby Street Medford MA 02155 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J. F.","family":"Nieland","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering School of Engineering Tufts University Science &amp; Technology Center  4 Colby Street Medford MA 02155 USA"},{"name":"Initiative for Neural Science, Disease &amp; Engineering Tufts University Science &amp; Engineering Complex  200 College Avenue Medford MA 02155 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9245-7774","authenticated-orcid":false,"given":"David L.","family":"Kaplan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering School of Engineering Tufts University Science &amp; Technology Center  4 Colby Street Medford MA 02155 USA"},{"name":"Initiative for Neural Science, Disease &amp; Engineering Tufts University Science &amp; Engineering Complex  200 College Avenue Medford MA 02155 USA"},{"name":"The Allen Discovery Center at Tufts University Biology Department Tufts University  200 Boston Ave. Suite 4600 Medford MA 02155 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,17]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402680101"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2007.12.053"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19970728)384:2<312::AID-CNE10>3.0.CO;2-K"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/13154"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/87565649109540500"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdrb.20090"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0531-5565(02)00151-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410210603"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awp007"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(16)30235-6"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2014.11.003"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/ab17aa"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.091"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1324214111"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2016.07.017"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm034327e"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2007.05.013"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.5b00446"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.8b00622"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.64.3.354"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1159\/000100926"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mp.4001220"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2194"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3892\/br.2016.630"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410160404"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1379.008"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12517"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914257107"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4707-08.2009"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4214"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.71.4.441"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34565"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201303196"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2012.09.007"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10821"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.7b00318"}],"container-title":["Macromolecular Bioscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmabi.202000004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mabi.202000004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mabi.202000004","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mabi.202000004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T01:28:38Z","timestamp":1694050118000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mabi.202000004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,17]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["10.1002\/mabi.202000004"],"URL":"https:\/\/doi.org\/10.1002\/mabi.202000004","archive":["Portico"],"relation":{},"ISSN":["1616-5187","1616-5195"],"issn-type":[{"value":"1616-5187","type":"print"},{"value":"1616-5195","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,17]]},"assertion":[{"value":"2020-01-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2000004"}}