{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T04:49:12Z","timestamp":1778820552019,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T00:00:00Z","timestamp":1592179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011176","name":"Peking Union Medical College","doi-asserted-by":"publisher","award":["201920201501"],"award-info":[{"award-number":["201920201501"]}],"id":[{"id":"10.13039\/501100011176","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin City","doi-asserted-by":"publisher","award":["19JCYBJC29300"],"award-info":[{"award-number":["19JCYBJC29300"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970769"],"award-info":[{"award-number":["81970769"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dry eye (DE) is a highly prevalent ocular surface disease which affects the quality of life and results in low working efficiency. Frequent instillation is required due to low bioavailability of conventional eye drops. The aim of this study is to develop a novel formulation of tacrolimus (TAC), routinely prescribed for DE, by combination of the microcrystal technology and layer\u2010by\u2010layer assembly. First, nonspherical tacrolimus microcrystals (TAC MCs) are synthesized by antisolvent\u2010induced precipitation. These TAC MCs are modified by alternate deposition of poly(allylamine hydrochloride) (PAH) and carboxymethyl cellulose (CMC) subsequently to obtain CMC\u2010coated TAC MCs (TAC\u2010(PAH\/CMC)<jats:sub>3<\/jats:sub>). The resultant formulations are evaluated in vivo in a mouse DE model induced by an intelligently controlled environmental system. Compared with commercially available TAC eye drops and the TAC MCs counterpart, TAC\u2010(PAH\/CMC)<jats:sub>3<\/jats:sub> exhibits superior therapeutic performance with reduced drug instillation frequency, which is attributed to the nonspherical geometry of MCs, the lubricant, mucoadhesive effect of CMC, and the anti\u2010inflammatory function of TAC. Therefore, TAC\u2010(PAH\/CMC)<jats:sub>3<\/jats:sub> represents a better option for the management of DE.<\/jats:p>","DOI":"10.1002\/mabi.202000079","type":"journal-article","created":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T08:24:55Z","timestamp":1592209495000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Carboxymethyl Cellulose\u2010Coated Tacrolimus Nonspherical Microcrystals for Improved Therapeutic Efficacy of Dry Eye"],"prefix":"10.1002","volume":"20","author":[{"given":"Caijie","family":"Zhang","sequence":"first","affiliation":[{"name":"Tianjin International Joint Research and Development Centre of Ophthalmology and Vision Science Eye Institute and School of Optometry Tianjin Medical University Eye Hospital  Tianjin 300384 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingying","family":"Zheng","sequence":"additional","affiliation":[{"name":"Tianjin Hospital  Tianjin 300211 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Li","sequence":"additional","affiliation":[{"name":"Biomedical Barriers Research Center Institute of Biomedical Engineering Chinese Academy of Medical Sciences &amp; Peking Union Medical College Tianjin Key Laboratory of Biomedical Materials  Tianjin 300192 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongfang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tianjin International Joint Research and Development Centre of Ophthalmology and Vision Science Eye Institute and School of Optometry Tianjin Medical University Eye Hospital  Tianjin 300384 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lianqing","family":"Chang","sequence":"additional","affiliation":[{"name":"Tianjin International Joint Research and Development Centre of Ophthalmology and Vision Science Eye Institute and School of Optometry Tianjin Medical University Eye Hospital  Tianjin 300384 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingyue","family":"Ai","sequence":"additional","affiliation":[{"name":"Biomedical Barriers Research Center Institute of Biomedical Engineering Chinese Academy of Medical Sciences &amp; Peking Union Medical College Tianjin Key Laboratory of Biomedical Materials  Tianjin 300192 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjie","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology Optometry and Vision Science School of Ophthalmology and Optometry School of Biomedical Engineering Wenzhou Medical University  Wenzhou 325027 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaozhen","family":"Zhao","sequence":"additional","affiliation":[{"name":"Tianjin International Joint Research and Development Centre of Ophthalmology and Vision Science Eye Institute and School of Optometry Tianjin Medical University Eye Hospital  Tianjin 300384 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Li","sequence":"additional","affiliation":[{"name":"Biomedical Barriers Research Center Institute of Biomedical Engineering Chinese Academy of Medical Sciences &amp; Peking Union Medical College Tianjin Key Laboratory of Biomedical Materials  Tianjin 300192 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4832-3530","authenticated-orcid":false,"given":"Zhimin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Biomedical Barriers Research Center Institute of Biomedical Engineering Chinese Academy of Medical Sciences &amp; Peking Union Medical College Tianjin Key Laboratory of Biomedical Materials  Tianjin 300192 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6,15]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2017.05.008"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/OPTH.S5555"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2017.05.006"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.9b13851"},{"key":"e_1_2_7_3_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2017.04.001"},{"key":"e_1_2_7_3_4","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2015.07.007"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2015.02.019"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2017.12.028"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2019.03.022"},{"key":"e_1_2_7_5_3","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1007\/s13346-016-0288-9","volume":"6","author":"Sharma O. P.","year":"2016","journal-title":"Drug Delivery Transl. Res."},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2015.02.039"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2007.70"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.04.011"},{"key":"e_1_2_7_7_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.05.024"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2017.06.038"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2009.06.020"},{"key":"e_1_2_7_8_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2014.01.041"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.6b00054"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2014.07.010"},{"key":"e_1_2_7_9_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2016.01.014"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2013-304453"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201300220"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matlet.2018.11.082"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.07-0744"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2017.05.001"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-010-0323-3"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2008.12.039"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/btm2.10122"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1jm11193k"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.201400405"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2010.02.013"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2016.09.067"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2017.05.011"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2014.05.001"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.120.3.330"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2007.131722"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2016.04.008"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2009.03.015"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.18-24504"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICL.0000000000000042"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.2001.1054"}],"container-title":["Macromolecular Bioscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmabi.202000079","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mabi.202000079","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mabi.202000079","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mabi.202000079","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T04:30:21Z","timestamp":1693888221000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mabi.202000079"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,15]]},"references-count":41,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,7]]}},"alternative-id":["10.1002\/mabi.202000079"],"URL":"https:\/\/doi.org\/10.1002\/mabi.202000079","archive":["Portico"],"relation":{},"ISSN":["1616-5187","1616-5195"],"issn-type":[{"value":"1616-5187","type":"print"},{"value":"1616-5195","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,15]]},"assertion":[{"value":"2020-03-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2000079"}}