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Anti\u2010CCR4 antibodies, which are utilized as sensing biomolecules, are covalently attached on the succinimide groups of polypyrrole polymer (<jats:italic>PPyr\u2010CSsg<\/jats:italic>). The constructed immunosensor illustrates promising performances for the quantification of CCR4 antigen, with a linear detection range of 0.024\u201312 pg mL<jats:sup>\u22121<\/jats:sup> and a low detection limit of 7.3 fg mL<jats:sup>\u22121<\/jats:sup>, calculated at a signal\u2010to\u2010noise ratio of 3. In addition, the impedimetric immunosensor displays a very successful analytical performance in terms of sensitivity, selectivity, repeatability, reproducibility, and long\u2010term stability as well as successful applicability for the accurate quantification of CCR4 in human serum samples. The constructed immunosensor is successfully used for quantification of CCR4 antigen in human serums. In addition, the immunosensor displays only 27.54% loss in its initial signal after nine weeks storage at 4\u00a0\u00b0C. Moreover, the fabricated immunosensor is economical, highly sensitive, and selective for CCR4 antigen detection, and suitable for potential application in clinical diagnosis.<\/jats:p>","DOI":"10.1002\/mabi.202000267","type":"journal-article","created":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T17:57:20Z","timestamp":1603821440000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Electrochemical Immunosensor for Detection of CCR4 Cancer Biomarker in Human Serum: An Alternative Strategy for Modification of Disposable ITO Electrode"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1982-4128","authenticated-orcid":false,"given":"Elif Burcu","family":"Ayd\u0131n","sequence":"first","affiliation":[{"name":"Scientific and Technological Research Center Tekirda\u011f Nam\u0131k Kemal University  Tekirda\u011f 59100 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