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A glass surface is coated with a thermoresponsive statistical copolymer of poly(N\u2010isopropylacrylamide\u2010co\u2010acrylamide) [p(NIPAm\u2010co\u2010Am)], which is end\u2010capped with the Gly\u2010Arg\u2010Gly\u2010Asp\u2010Ser (GRGDS) peptide, and the hydrophilic polymer poly(ethylene glycol) (PEG). Below the lower critical solution temperature (LCST) of p(NIPAm\u2010co\u2010Am) (38 \u00b0C), the copolymers are in the extended conformation, allowing for accessibility of the GRGDS peptides to membrane\u2010associated integrins thus enabling cell attachment. Above the LCST, the p(NIPAm\u2010co\u2010Am) polymers collapse into globular conformations, resulting in the shielding of the GRGDS peptides into the PEG brush with consequent inaccessibility to cell\u2010surface integrins, causing cell detachment. The surface coating is carried out by a multi\u2010step procedure that included: glass surface amination with 3\u2010aminopropyltriethoxysilane; reaction of mPEG<jats:sub>5kDa<\/jats:sub>\u2010N\u2010hydroxysuccinimide (NHS) and p(NIPam\u2010co\u2010Am)<jats:sub>15.1kDa<\/jats:sub>\u2010bis\u2010NHS with the surface aminopropyl groups and conjugation of GRGDS to the carboxylic acid termini of p(NIPam\u2010co\u2010Am)<jats:sub>15.1kDa<\/jats:sub>\u2010COOH. A range of spectrophotometric, surface, and microscopy assays confirmed the identity of the polymer\u2010coated substrates. Competition studies prove that MCF\u20107 cancer cells are attached via peptide recognition at the coated surfaces according to the mPEG<jats:sub>5kDa<\/jats:sub>\/p(NIPam\u2010co\u2010Am)<jats:sub>15.1kDa<\/jats:sub>\u2010GRGDS molar ratio. These data suggest the system can be exploited to modulate cell integrin\/GRGDS binding for controlled cell capture and release.<\/jats:p>","DOI":"10.1002\/mabi.202000277","type":"journal-article","created":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T18:33:34Z","timestamp":1604514814000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Thermosensitive \u201cSmart\u201d Surfaces for Biorecognition Based Cell Adhesion and Controlled Detachment"],"prefix":"10.1002","volume":"21","author":[{"given":"Silvia","family":"Brunato","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences University of Padova  via F. 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