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This phenomenon, termed fouling, is then followed by a series of undesired reactions involving activation of complement or the coagulation cascade and adhesion of leukocytes and platelets leading to thrombus formation. Thus, considerable efforts are directed towards the preparation of fouling\u2010resistant surfaces with the best possible hemocompatibility. Herein, a comprehensive hemocompatibility study after heparinized blood contact with seven polymer brushes prepared by surface\u2010initiated atom transfer radical polymerization is reported. The resistance to fouling is quantified and thrombus formation and deposition of blood cellular components on the coatings are analyzed. Moreover, identification of the remaining adsorbed proteins is performed via mass spectroscopy to elucidate their influence on the surface hemocompatibility. Compared with an unmodified glass surface, the grafting of polymer brushes minimizes the adhesion of platelets and leukocytes and prevents the thrombus formation. The fouling from undiluted blood plasma is reduced by up to 99%. Most of the identified proteins are connected with the initial events of foreign body reaction towards biomaterial (coagulation cascade proteins, complement component, and inflammatory proteins). In addition, several proteins that are not previously linked with blood\u2010biomaterial interaction are presented and discussed.<\/jats:p>","DOI":"10.1002\/mabi.202200247","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T15:45:39Z","timestamp":1659455139000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["The Relation Between Protein Adsorption and Hemocompatibility of Antifouling Polymer Brushes"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5614-1871","authenticated-orcid":false,"given":"Zuzana","family":"Riedelov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Macromolecular Chemistry Czech Academy of Sciences  Heyrovsk\u00e9ho n\u00e1m. 2 Prague 162 06 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0138-1357","authenticated-orcid":false,"given":"Andres","family":"de los Santos Pereira","sequence":"additional","affiliation":[{"name":"Institute of Macromolecular Chemistry Czech Academy of Sciences  Heyrovsk\u00e9ho n\u00e1m. 2 Prague 162 06 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4989-4274","authenticated-orcid":false,"given":"Jan","family":"Svoboda","sequence":"additional","affiliation":[{"name":"Institute of Macromolecular Chemistry Czech Academy of Sciences  Heyrovsk\u00e9ho n\u00e1m. 2 Prague 162 06 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7938-9271","authenticated-orcid":false,"given":"Ognen","family":"Pop\u2010Georgievski","sequence":"additional","affiliation":[{"name":"Institute of Macromolecular Chemistry Czech Academy of Sciences  Heyrovsk\u00e9ho n\u00e1m. 2 Prague 162 06 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9946-3819","authenticated-orcid":false,"given":"Pavel","family":"M\u00e1jek","sequence":"additional","affiliation":[{"name":"Institute of Hematology and Blood Transfusion  U Nemocnice 1 Prague 128 00 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kl\u00e1ra","family":"Pe\u010d\u00e1nkov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Hematology and Blood Transfusion  U Nemocnice 1 Prague 128 00 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9687-2150","authenticated-orcid":false,"given":"Filip","family":"Dy\u010dka","sequence":"additional","affiliation":[{"name":"Faculty of Science University of South Bohemia  Brani\u0161ovsk\u00e1 1760 \u010cesk\u00e9 Bud\u011bjovice 370 05 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0745-0840","authenticated-orcid":false,"given":"Cesar","family":"Rodriguez\u2010Emmenegger","sequence":"additional","affiliation":[{"name":"Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST) Carrer de Baldiri Reixac  10, 12 Barcelona 08028 Spain"},{"name":"Instituci\u00f3 Catalana de Recerca i Estudis Avan\u00e7ats (ICREA)  Passeig Llu\u00eds Companys 23 Barcelona 08010 Spain"},{"name":"DWI\u2013Leibniz Institute for Interactive Materials  Forckenbeckstra\u00dfe 50 D\u201052074 Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2971-6956","authenticated-orcid":false,"given":"Tom\u00e1\u0161","family":"Riedel","sequence":"additional","affiliation":[{"name":"Institute of Macromolecular Chemistry Czech Academy of Sciences  Heyrovsk\u00e9ho n\u00e1m. 2 Prague 162 06 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,9]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/admi.201700954"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820270302"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.07.035"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.06.021"},{"key":"e_1_2_8_5_1","first-page":"E166","volume":"7","author":"Makdisi G.","year":"2015","journal-title":"J. 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