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Towards this direction, four diblock copolymers comprising polyethylene glycol (PEG) and poly(2\u2010tetrahydropyranyl methacrylate) (PTHPMA) are synthesized employing PEG macroinitiators of different chain lengths. The copolymer with a 5000\u00a0Da PEG block and a PEG\u2010PTHPMA comonomers weight ratio of 43\u201357 provides a film with the highest stability in the culture medium and the strongest cell repellent properties. This copolymer is used to develop a positive photolithographic material and create stripe patterns onto silicon substrates. The highest selectivity regarding smooth muscle cell adhesion and growth and the highest fidelity of adhered cells for up to 3 days in culture is achieved for stripe patterns with widths between 25 and 27.5\u00a0\u00b5m. Smooth muscle cells cultured on such patterned substrates exhibit a decrease in their proliferation rate and nucleus area and an increase in their major axis length, compared to the cells cultured onto non\u2010patterned substrates. These alterations are indicative of the adoption of a contractile rather than a synthetic phenotype of the smooth muscle cells grown onto the patterned substrates and demonstrate the potential of the novel photolithographic material and patterning method for guided cell adhesion and growth.<\/jats:p>","DOI":"10.1002\/mabi.202200301","type":"journal-article","created":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T10:29:45Z","timestamp":1664792985000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Photolithographically Patterned Cell\u2010Repellent PEG\u2010<i>b<\/i>\u2010PTHPMA Diblock Copolymer for Guided Cell Adhesion and Growth"],"prefix":"10.1002","volume":"23","author":[{"given":"Dimitra","family":"Kourti","sequence":"first","affiliation":[{"name":"Immunoassays\u2010Immunosensors Lab Institute of Nuclear &amp; Radiological Sciences &amp; Technology Energy &amp; Safety NCSR \u201cDemokritos\u201d  Aghia Paraskevi 15341 Greece"},{"name":"Department of Chemistry University of Athens  Panepistimiopolis Zografou 15771 Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Kanioura","sequence":"additional","affiliation":[{"name":"Immunoassays\u2010Immunosensors Lab Institute of Nuclear &amp; 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