{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:15:15Z","timestamp":1792181715480,"version":"4.4.0"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T00:00:00Z","timestamp":1670544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["CSC201906010329"],"award-info":[{"award-number":["CSC201906010329"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["ERC\u20102014\u2010StG\u2010637830"],"award-info":[{"award-number":["ERC\u20102014\u2010StG\u2010637830"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Polyethylenimine (PEI) is a commonly used cationic polymer for small\u2010interfering RNA (siRNA) delivery due to its high transfection efficiency at low commercial cost. However, high molecular weight PEI is cytotoxic and thus, its practical application is limited. In this study, different formulations of low molecular weight PEI (LMW\u2010PEI) based copolymers polyethylenimine\u2010\n                    <jats:italic>g<\/jats:italic>\n                    \u2010polycaprolactone (PEI\u2013PCL) (800\u00a0Da\u201340\u00a0kDa) and PEI\u2013PCL\u2013PEI (5\u20135\u20135\u00a0kDa) blended with or without polyethylene glycol\u2010\n                    <jats:italic>b<\/jats:italic>\n                    \u2010polycaprolactone (PEG\u2013PCL) (5\u00a0kDa\u20104\u00a0kDa) are investigated to prepare nanoparticles via nanoprecipitation using a solvent displacement method with sizes \u2248100\u00a0nm. PEG\u2013PCL can stabilize the nanoparticles, improve their biocompatibility, and extend their circulation time in vivo. The nanoparticles composed of PEI\u2013PCL\u2013PEI and PEG\u2013PCL show higher siRNA encapsulation efficiency than PEI\u2013PCL\/PEG\u2013PCL based nanoparticles at low N\/P ratios, higher cellular uptake, and a gene silencing efficiency of \u224840% as a result of the higher molecular weight PEI blocks. These results suggest that the PEI\u2013PCL\u2013PEI\/PEG\u2013PCL nanoparticle system could be a promising vehicle for siRNA delivery at minimal synthetic effort.\n                  <\/jats:p>","DOI":"10.1002\/mabi.202200409","type":"journal-article","created":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T01:00:25Z","timestamp":1669770025000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Synthesis and Application of Low Molecular Weight PEI\u2010Based Copolymers for siRNA Delivery with Smart Polymer Blends"],"prefix":"10.1002","volume":"23","author":[{"given":"Yao","family":"Jin","sequence":"first","affiliation":[{"name":"Department of Pharmacy Ludwig\u2010Maximilians\u2010University Munich Pharmaceutical Technology and Biopharmaceutics  Butenandtstr. 5\u201313 81377 Munich 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