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Thiol\u2010mediated conjugation of biomolecules is shown to be quite advantageous for such purposes due to the reactivity and chemoselectivity of thiol functional groups. Herein, the immobilization of poly(ethylene glycol) (PEG) and antimicrobial peptides (AMPs) to silica colloidal particles based on thiol\u2010mediated conjugation techniques, along with an assessment of the antimicrobial potential of the functionalized particles against <jats:italic>Pseudomonas aeruginosa<\/jats:italic> and <jats:italic>Staphylococcus aureus<\/jats:italic> is investigated. Immobilization of PEG to thiolated Si particles is performed by either a two\u2010step thiol\u2013ene \u201cphoto\u2010click\u201d reaction or a \u201cone\u2010pot\u201d thiol\u2013maleimide type conjugation using terminal acrylate or maleimide functional groups, respectively. It is demonstrated that both immobilization methods result in a significant reduction in the number of viable bacterial cells compared to unmodified samples after the designated incubation periods with the PEG\u2010AMP\u2010modified colloidal suspensions. These findings provide a promising outlook for the fabrication of multifunctional surfaces based upon the tethering of PEG and AMPs to colloidal particles through thiol\u2010mediated biocompatible chemistry, which has potential for use as implant coatings or as antibacterial formulations that can be incorporated into wound dressings to prevent or control bacterial infections.<\/jats:p>","DOI":"10.1002\/mabi.202200411","type":"journal-article","created":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T21:39:51Z","timestamp":1683841191000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Enhancing the Activity of Surface Immobilized Antimicrobial Peptides Using Thiol\u2010Mediated Tethering to Poly(ethylene glycol)"],"prefix":"10.1002","volume":"23","author":[{"given":"Andrew","family":"Boden","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Science, Computing and Engineering Technologies Swinburne University of Technology  Hawthorn VIC 3122 Australia"},{"name":"ARC Training Centre in Surface Engineering for Advanced Materials (SEAM), School of Engineering Swinburne University of Technology  Hawthorn VIC 3122 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Dart","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Science, Computing and Engineering Technologies Swinburne University of Technology  Hawthorn VIC 3122 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu\u2010Ying","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Science, Computing and Engineering Technologies Swinburne University of Technology  Hawthorn VIC 3122 Australia"},{"name":"ARC Training Centre in Surface Engineering for Advanced Materials (SEAM), School of Engineering Swinburne University of Technology  Hawthorn VIC 3122 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De Ming","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Science, Computing and Engineering Technologies Swinburne University of Technology  Hawthorn VIC 3122 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mrinal","family":"Bhave","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Science, Computing and Engineering Technologies Swinburne University of Technology  Hawthorn VIC 3122 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Cipolla","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biosciences University of Milano \u2013 Bicocca  Piazza della Scienza 2 Milano 20126 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5882-5804","authenticated-orcid":false,"given":"Peter","family":"Kingshott","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Science, Computing and Engineering Technologies Swinburne University of Technology  Hawthorn VIC 3122 Australia"},{"name":"ARC Training Centre in Surface Engineering for Advanced Materials (SEAM), School of Engineering Swinburne University of Technology  Hawthorn VIC 3122 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,5,24]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4TB01905A"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.iecr.5b02864"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.5b12660"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cis.2018.08.005"},{"key":"e_1_2_8_5_1","volume":"6","author":"Guggenbichler J. 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