{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T08:59:53Z","timestamp":1778403593067,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,11]],"date-time":"2022-12-11T00:00:00Z","timestamp":1670716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["422041745"],"award-info":[{"award-number":["422041745"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010664","name":"H2020 Future and Emerging Technologies","doi-asserted-by":"publisher","award":["731957"],"award-info":[{"award-number":["731957"]}],"id":[{"id":"10.13039\/100010664","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Hydrogel precursors that crosslink within minutes are essential for the development of cell encapsulation matrices and their implementation in automated systems. Such timescales allow sufficient mixing of cells and hydrogel precursors under low shear forces and the achievement of homogeneous networks and cell distributions in the 3D cell culture. The previous work showed that the thiol\u2010tetrazole methylsulfone (TzMS) reaction crosslinks star\u2010poly(ethylene glycol) (PEG) hydrogels within minutes at around physiological pH and can be accelerated or slowed down with small pH changes. The resulting hydrogels are cytocompatible and stable in cell culture conditions. Here, the gelation kinetics and mechanical properties of PEG\u2010based hydrogels formed by thiol\u2010TzMS crosslinking as a function of buffer, crosslinker structure and degree of TzMS functionality are reported. Crosslinkers of different architecture, length and chemical nature (PEG versus peptide) are tested, and degree of TzMS functionality is modified by inclusion of RGD cell\u2010adhesive ligand, all at concentration ranges typically used in cell culture. These studies corroborate that thiol\/PEG\u20104TzMS hydrogels show gelation times and stiffnesses that are suitable for 3D cell encapsulation and tunable through changes in hydrogel composition. The results of this study guide formulation of encapsulating hydrogels for manual and automated 3D cell culture.<\/jats:p>","DOI":"10.1002\/mabi.202200419","type":"journal-article","created":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T02:59:19Z","timestamp":1669949959000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Gelation Kinetics and Mechanical Properties of Thiol\u2010Tetrazole Methylsulfone Hydrogels Designed for Cell Encapsulation"],"prefix":"10.1002","volume":"23","author":[{"given":"Adri\u00e1n","family":"de Miguel\u2010Jim\u00e9nez","sequence":"first","affiliation":[{"name":"INM \u2013 Leibniz Institute for New Materials  Campus D2 2 66123 Saarbr\u00fccken Germany"},{"name":"Chemistry Department Saarland University  66123 Saarbr\u00fccken Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bastian","family":"Ebeling","sequence":"additional","affiliation":[{"name":"Kuraray Europe GmbH Advanced Interlayer Solutions Competence Center for Innovation &amp; Technology  M\u00fclheimer Str. 26 53840 Troisdorf Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julieta I.","family":"Paez","sequence":"additional","affiliation":[{"name":"INM \u2013 Leibniz Institute for New Materials  Campus D2 2 66123 Saarbr\u00fccken Germany"},{"name":"Current address: Department of Developmental BioEngineering Technical Medical Centre University of Twente  Enschede The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Fink\u2010Straube","sequence":"additional","affiliation":[{"name":"INM \u2013 Leibniz Institute for New Materials  Campus D2 2 66123 Saarbr\u00fccken Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4663-2025","authenticated-orcid":false,"given":"Samuel","family":"Pearson","sequence":"additional","affiliation":[{"name":"INM \u2013 Leibniz Institute for New Materials  Campus D2 2 66123 Saarbr\u00fccken Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ar\u00e1nzazu","family":"del Campo","sequence":"additional","affiliation":[{"name":"INM \u2013 Leibniz Institute for New Materials  Campus D2 2 66123 Saarbr\u00fccken Germany"},{"name":"Chemistry Department Saarland University  66123 Saarbr\u00fccken 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