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In this study, multi\u2010block copolymers composed of poly(2\u2010methoxyethyl acrylate) (PMEA) and polyethylene glycol (PEG) are developed as a new kind of biocompatible polymers. PEG imparts non\u2010cell adhesion while PMEA acts as an anchoring segment to prepare the polymer coating surface. The multi\u2010block copolymers show higher stability in water than PMEA. A specific micro\u2010sized swelling structure composed of a PEG chain is observed in the multi\u2010block copolymer film in water. A single NIH3T3\u20103\u20104 spheroid is formed in 3\u00a0h on the surface of the multi\u2010block copolymers with 8.4 wt% PEG. However, at a PEG content of 0.7 wt%, spheroid formed after 4 days. The adenosine triphosphate (ATP) activity of cells and the internal necrotic state of the spheroid change depending on PEG loading in the multi\u2010block copolymers. As the formation rate of cell spheroid on low\u2010PEG\u2010ratio multi\u2010block copolymers is slow, internal necrosis of cell spheroid is less likely to occur. Consequently, the cell spheroid formation rate by changing the PEG chain content in multi\u2010block copolymers is successfully controlled. These unique surfaces are suggested to be useful for 3D cell culture.<\/jats:p>","DOI":"10.1002\/mabi.202200486","type":"journal-article","created":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T07:46:47Z","timestamp":1678175207000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cell Spheroid Formation on the Surface of Multi\u2010Block Copolymers Composed of Poly(2\u2010methoxyethyl acrylate) and Polyethylene Glycol"],"prefix":"10.1002","volume":"23","author":[{"given":"Naoto","family":"Ogiwara","sequence":"first","affiliation":[{"name":"Department of Bioengineering School of Engineering The University of Tokyo  7\u20103\u20101 Hongo Bunkyo\u2010ku Tokyo 113\u20108656 Japan"},{"name":"Corporate R&amp;D Division Toyo Ink SC Holdings Co., Ltd.  5\u20106\u20107 Chiyoda Sakado\u2010city Saitama 350\u20100214 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takenobu","family":"Nakano","sequence":"additional","affiliation":[{"name":"Corporate R&amp;D Division Toyo Ink SC Holdings Co., Ltd.  5\u20106\u20107 Chiyoda Sakado\u2010city Saitama 350\u20100214 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsukuru","family":"Masuda","sequence":"additional","affiliation":[{"name":"Department of Bioengineering School of Engineering The University of Tokyo  7\u20103\u20101 Hongo Bunkyo\u2010ku Tokyo 113\u20108656 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiichiro","family":"Kushiro","sequence":"additional","affiliation":[{"name":"Department of Bioengineering School of Engineering The University of Tokyo  7\u20103\u20101 Hongo Bunkyo\u2010ku Tokyo 113\u20108656 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6238-6717","authenticated-orcid":false,"given":"Madoka","family":"Takai","sequence":"additional","affiliation":[{"name":"Department of Bioengineering School of Engineering The University of Tokyo  7\u20103\u20101 Hongo Bunkyo\u2010ku Tokyo 113\u20108656 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,12]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.117784"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13040874"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.226"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/C0AN00609B"},{"key":"e_1_2_8_5_1","author":"Xu F.","year":"2014","journal-title":"BioMed Res. 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