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However, the CAM has a short period in vivo, and the in vivo maintenance is not controlled. Therefore, this study is aimed at developing an injectable hydrogel scaffold using a CAM. The CAM is cross\u2010linked with a biocompatible polyethylene glycol (PEG) cross\u2010linker to replace typically used glutaraldehyde (GA) cross\u2010linker. The cross\u2010linking degree of cross\u2010linked CAM by PEG cross\u2010linker (Cx\u2010CAM\u2010PEG) according to the ratios of the CAM and PEG cross\u2010linker is confirmed by contact angle and heat capacities measured by differential scanning calorimetry. The injectable Cx\u2010CAM\u2010PEG suspension exhibits controllable rheological properties and injectability. Additionally, injectable Cx\u2010CAM\u2010PEG suspensions with no free aldehyde group are formed in the in vivo hydrogel scaffold almost simultaneously with injection. In vivo maintenance of Cx\u2010CAM\u2010PEG is realized by the cross\u2010linking ratio. The in vivo formed Cx\u2010CAM\u2010PEG hydrogel scaffold exhibits certain host\u2013cell infiltration and negligible inflammation within and near the transplanted Cx\u2010CAM\u2010PEG hydrogel scaffold. These results suggest that injectable Cx\u2010CAM\u2010PEG suspensions, which are safe and biocompatible in vivo, represent potential candidates for (pre\u2010)clinical scaffolds.<\/jats:p>","DOI":"10.1002\/mabi.202300029","type":"journal-article","created":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T13:25:34Z","timestamp":1680009934000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Development and In Vivo Assessment of an Injectable Cross\u2010Linked Cartilage Acellular Matrix\u2010PEG Hydrogel Scaffold Derived from Porcine Cartilage for Tissue Engineering"],"prefix":"10.1002","volume":"23","author":[{"given":"Hyeon Jin","family":"Ju","sequence":"first","affiliation":[{"name":"Department of Molecular Science and Technology Ajou University  Suwon 443\u2013749 South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun 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