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Photocurable hyaluronic acid (HA) hydrogel (AHAMA\u2010PBA) modified with 3\u2010aminophenylboronic acid, sodium periodate, and methacrylic anhydride simultaneously is constructed in this study. Chondrocytes are then cultured on the surface of the hydrogels to evaluate the effect of the physicochemical properties of the hydrogels on modulating cellular behaviors. Cell viability assays demonstrate that the hydrogel is non\u2010toxic to chondrocytes. The existence of phenylboronic acid (PBA) moieties enhances the interaction of chondrocytes and hydrogel, promoting cell adhesion and aggregation through filopodia. RT\u2010PCR indicates that the gene expression levels of type II collagen, Aggrecan, and Sox9 are significantly up\u2010regulated in chondrocytes cultured on hydrogels. Moreover, the mechanical properties of the hydrogels have a significant effect on the cell phenotype, with soft gels (\u22482\u00a0kPa) promoting chondrocytes to exhibit a hyaline phenotype. Overall, PBA\u2010functionalized HA hydrogel with low stiffness exhibits the best effect on promoting the chondrocyte phenotype, which is a promising biomaterial for cartilage regeneration.<\/jats:p>","DOI":"10.1002\/mabi.202300153","type":"journal-article","created":{"date-parts":[[2023,7,3]],"date-time":"2023-07-03T23:33:10Z","timestamp":1688427190000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Bioactive Phenylboronic Acid\u2010Functionalized Hyaluronic Acid Hydrogels Induce Chondro\u2010Aggregates and Promote Chondrocyte Phenotype"],"prefix":"10.1002","volume":"23","author":[{"given":"Ying","family":"Liu","sequence":"first","affiliation":[{"name":"School of Materials Science and Engineering, National Engineering Research Center for Tissue Restoration and Reconstruction, Key Laboratory of Biomedical Engineering of Guangdong Province, Key Laboratory of Biomedical Materials and Engineering of the Ministry of 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