{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T20:28:48Z","timestamp":1778704128603,"version":"3.51.4"},"reference-count":69,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T00:00:00Z","timestamp":1695859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012021","name":"Iran University of Medical Sciences","doi-asserted-by":"publisher","award":["IUMS 1400\u201003\u201004\u201022039"],"award-info":[{"award-number":["IUMS 1400\u201003\u201004\u201022039"]}],"id":[{"id":"10.13039\/100012021","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Numerous scaffolds are developed in the field of testicular bioengineering. However, effectively replicating the spatial characteristics of native tissue, poses a challenge in maintaining the requisite cellular arrangement essential for spermatogenesis. In order to mimic the structural properties of seminiferous tubules, the objective is to fabricate a biocompatible tubular scaffold. Following the decellularization process of the testicular tissue, validation of cellular remnants' elimination from the specimens is conducted using 4\u2032,6\u2010diamidino\u20102\u2010phenylindole staining, hematoxylin and eosin staining, and DNA content analysis. The presence of extracellular matrix (ECM) components is confirmed through Alcian blue, Orcein, and Masson's trichrome staining techniques. The electrospinning technique is employed to synthesize the scaffolds using polycaprolactone (PCL), extracted ECM, and varying concentrations of graphene oxide (GO) (0.5%, 1%, and 2%). Subsequently, comprehensive evaluations are performed to assess the properties of the synthetic scaffolds. These evaluations encompass Fourier\u2010transform infrared spectroscopy, scanning electron microscopy imaging, scaffold degradation testing, mechanical behavior analysis, methylthiazolyldiphenyl\u2010tetrazolium bromide assay, and in vivo biocompatibility assessment. The PCL\/decellularized extracellular matrix with 0.5% GO formulation exhibits superior fiber morphology and enhanced mechanical properties, and outperforms other groups in terms of in vitro biocompatibility. Consequently, these scaffolds present a viable option for implementation in \u201cin vitro spermatogenesis\u201d procedures, holding promise for future sperm production from spermatogonial cells.<\/jats:p>","DOI":"10.1002\/mabi.202300342","type":"journal-article","created":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T22:59:01Z","timestamp":1695250741000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Polycaprolactone\/Testicular Extracellular Matrix\/Graphene Oxide\u2010Based Electrospun Tubular Scaffolds for Reproductive Medicine: Biomimetic Architecture of Seminiferous Tubules"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2813-3986","authenticated-orcid":false,"given":"Amirhossein","family":"Mohammadi","sequence":"first","affiliation":[{"name":"Stem Cell and Regenerative Medicine Research Center Iran University of Medical Sciences Tehran 88770048 Iran"},{"name":"Department of Anatomy School of Medicine Iran University of Medical Sciences Tehran 1449614525 Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morteza","family":"Koruji","sequence":"additional","affiliation":[{"name":"Stem Cell and Regenerative Medicine Research Center Iran University of Medical Sciences Tehran 88770048 Iran"},{"name":"Department of Anatomy School of Medicine Iran University of Medical Sciences Tehran 1449614525 Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmoud","family":"Azami","sequence":"additional","affiliation":[{"name":"Department of Tissue Engineering School of Advanced Technologies in Medicine Tehran University of Medical Sciences Tehran 1417755469 Iran"},{"name":"Joint Reconstruction Research Center (JRRC) Tehran University of Medical Sciences Tehran 1417755469 Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronak","family":"Shabani","sequence":"additional","affiliation":[{"name":"Reproductive Sciences and Technology Research Center Department of Anatomy School of Medicine Iran University of Medical Sciences Tehran 1449614535 Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanam","family":"Mohandesnezhad","sequence":"additional","affiliation":[{"name":"Department of Tissue Engineering School of Advanced Technologies in Medicine Tehran University of Medical Sciences Tehran 1417755469 Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Bashiri","sequence":"additional","affiliation":[{"name":"Department of Anatomy School of Medicine Iran University of Medical Sciences Tehran 1449614525 Iran"},{"name":"Endometrium and Endometriosis Research Center Hamadan University of Medical Sciences Hamadan 6517789971 Iran"},{"name":"Omid Fertility &amp; Infertility Clinic Hamedan 6516796198 Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1864-0909","authenticated-orcid":false,"given":"Hamidreza","family":"Asgari","sequence":"additional","affiliation":[{"name":"Stem Cell and Regenerative Medicine Research Center Iran University of Medical Sciences Tehran 88770048 Iran"},{"name":"Department of Anatomy School of Medicine Iran University of Medical Sciences Tehran 1449614525 Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,9,28]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmv016"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.11.037"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/1008-682X.148719"},{"key":"e_1_2_10_4_1","first-page":"62","volume":"12","author":"Esteves S.","year":"2018","journal-title":"Anim. 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