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Herein the synthesis of ionic and degradable hydrogels is demonstrated for cell culture by crosslinking [PEG\u2010SH]<jats:sub>4<\/jats:sub> with the zwitterionic crosslinker <jats:italic>N,N<\/jats:italic>\u2010bis(acryloxyethyl)\u2010<jats:italic>N<\/jats:italic>\u2010methyl\u2010<jats:italic>N<\/jats:italic>\u2010(3\u2010sulfopropyl) ammonium betaine (BMSAB) and the cationic crosslinker <jats:italic>N,N<\/jats:italic>\u2010bis(acryloxyethyl)\u2010<jats:italic>N,N<\/jats:italic>\u2010dimethyl\u20101\u2010ammonium iodide (BDMAI). Depending on the amount of ionic crosslinker used in gel formation, the hydrogels show tunable gelation time and stiffness. At the same time, the ionic groups act as catalysts for hydrolytic degradation, thereby allowing to define a stability window. The latter could be tailored in a straightforward manner by introducing the non\u2010degradable crosslinker tri(ethylene glycol) divinyl ether. In addition, both ionic crosslinkers favor cell attachment in comparison to the pristine PEG hydrogels. The degradation is examined by swelling behavior, rheology, and fluorescence correlation spectroscopy indicating degradation kinetics depending on diffusion of incorporated fluorescent molecules.<\/jats:p>","DOI":"10.1002\/mabi.202300383","type":"journal-article","created":{"date-parts":[[2023,12,16]],"date-time":"2023-12-16T13:31:36Z","timestamp":1702733496000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Self\u2010Degrading Multifunctional PEG\u2010Based Hydrogels\u2014Tailormade Substrates for Cell Culture"],"prefix":"10.1002","volume":"24","author":[{"given":"Kathrin","family":"Kowalczuk","sequence":"first","affiliation":[{"name":"Institute of Organic Chemistry and Macromolecular Chemistry (IOMC) Friedrich\u2010Schiller\u2010University Jena  Lessingstra\u00dfe 8 07743 Jena Germany"},{"name":"Jena Center for Soft Matter (JCSM) Friedrich\u2010Schiller\u2010University Jena  Philosophenweg 7 07743 Jena 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