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However, the slow gelation process observed in these cell\u2010encapsulating hydrogels often causes an uneven dispersion of cells. This study proposes an approach for achieving fast gelation of unmodified gelatin under physiological conditions through gelatin preclustering. By using tetrafunctional succinimidyl\u2010terminated poly(ethylene glycol) as a clustering agent, the gelation process is successfully expedited fivefold without requiring chemical modifications, effectively addressing the associated challenges of uneven cell distribution.<\/jats:p>","DOI":"10.1002\/mabi.202300450","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T04:14:22Z","timestamp":1708920862000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Preclustering Gelatin for Faster\u2010Forming Injectable Hydrogels: A Strategy for Fabricating 3D Hydrogel Scaffolds with Improved Cell Dispersion"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7442-9454","authenticated-orcid":false,"given":"Shohei","family":"Ishikawa","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Engineering The University of Tokyo 7\u20103\u20101 Hongo Bunkyo\u2010ku Tokyo 113\u20108656 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5933-8677","authenticated-orcid":false,"given":"Hiroyuki","family":"Kamata","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Engineering The University of Tokyo 7\u20103\u20101 Hongo Bunkyo\u2010ku Tokyo 113\u20108656 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5052-0512","authenticated-orcid":false,"given":"Takamasa","family":"Sakai","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biotechnology, School of Engineering The University of Tokyo 7\u20103\u20101 Hongo Bunkyo\u2010ku Tokyo 113\u20108656 Japan"},{"name":"Department of Bioengineering, School of Engineering The University of Tokyo 7\u20103\u20101 Hongo Bunkyo\u2010ku Tokyo 113\u20108656 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,3]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1214804"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00340-5"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/1087057117696795"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-018-0034-7"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/b713009k"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2014.07.006"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/b713009k"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(19990915)46:4<520::AID-JBM10>3.0.CO;2-9"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.02.001"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ma100176f"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemmater.9b02485"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13770-018-0152-8"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr500399p"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201604062"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-017-0044"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.1053"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.01703.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13367-013-0023-8"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ma302270a"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781315274386"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9SM00696F"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/9783527610969"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3136\/fstr.25.217"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/b908386c"},{"key":"e_1_2_8_25_1","volume-title":"Polymer Handbook","author":"Immergut J. 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