{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T13:05:31Z","timestamp":1793970331361,"version":"4.8.0"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,12,2]],"date-time":"2023-12-02T00:00:00Z","timestamp":1701475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52173124"],"award-info":[{"award-number":["52173124"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2172019kfyXJJS070"],"award-info":[{"award-number":["2172019kfyXJJS070"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Antimicrobial peptides (AMPs) potentially serve as ideal antimicrobial agents for the treatment of polymicrobial abdominal infections due to their broad\u2010spectrum antimicrobial activity and excellent biocompatibility. However, the balance of chain length, positive charges, and hydrophobicity on the antimicrobial activity of AMPs are still far from being optimal. Herein, a series of AMPs ([KX]<jats:sub><jats:italic>n<\/jats:italic><\/jats:sub>\u2010NH<jats:sub>2<\/jats:sub>, X = Ile, Leu or Phe, n = 3, 4, 5, or 6) with varied charges and hydrophobicity for the treatment of polymicrobial abdominal infections are designed. Specifically, [KI]<jats:sub>4<\/jats:sub>\u2010NH<jats:sub>2<\/jats:sub> peptide exhibits the best in vitro antimicrobial activity against Gram\u2010positive and \u2010negative bacteria, as well as fungal strains. Based on the good cell biocompatibility, [KI]<jats:sub>4<\/jats:sub>\u2010NH<jats:sub>2<\/jats:sub> peptide is found to have negligible in vivo toxicity at the dosage of up to 28\u00a0mg\u00a0kg<jats:sup>\u22121<\/jats:sup>. Furthermore, great in vivo therapeutic efficacy of [KI]<jats:sub>4<\/jats:sub>\u2010NH<jats:sub>2<\/jats:sub> peptide against <jats:italic>S. typhimurium<\/jats:italic> is demonstrated in the mice abdominal infection model. The design of short sequence of antimicrobial peptides with a charge\/hydrophobicity balanced structures provides a simple and efficient strategy for potential clinical applications of antimicrobial peptide\u2010based biomaterials in a variety of bacterial infection diseases.<\/jats:p>","DOI":"10.1002\/mabi.202300451","type":"journal-article","created":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T06:52:24Z","timestamp":1700808744000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Optimized Charge\/Hydrophobicity Balance of Antimicrobial Peptides Against Polymicrobial Abdominal Infections"],"prefix":"10.1002","volume":"24","author":[{"given":"Kehan","family":"Du","sequence":"first","affiliation":[{"name":"Hubei Engineering Research Center for Biomaterials and Medical Protective Materials School of Chemistry and Chemical Engineering Huazhong University of Science and Technology (HUST)  Wuhan 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo\u2010Ran","family":"Yang","sequence":"additional","affiliation":[{"name":"Hubei Engineering Research Center for Biomaterials and Medical Protective Materials School of Chemistry and Chemical Engineering Huazhong University of Science and Technology (HUST)  Wuhan 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Qin","sequence":"additional","affiliation":[{"name":"Hubei Engineering Research Center for Biomaterials and Medical Protective Materials School of Chemistry and Chemical Engineering Huazhong University of Science and Technology (HUST)  Wuhan 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teng","family":"Ma","sequence":"additional","affiliation":[{"name":"Hubei Engineering Research Center for Biomaterials and Medical Protective Materials School of Chemistry and Chemical Engineering Huazhong University of Science and Technology (HUST)  Wuhan 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawei","family":"Tang","sequence":"additional","affiliation":[{"name":"Hubei Engineering Research Center for Biomaterials and Medical Protective Materials School of Chemistry and Chemical Engineering Huazhong University of Science and Technology (HUST)  Wuhan 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianxin","family":"Xia","sequence":"additional","affiliation":[{"name":"Win Plus Biotechnology Co., Ltd.  Wuhan 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zengtai","family":"Zhou","sequence":"additional","affiliation":[{"name":"Win Plus Biotechnology Co., Ltd.  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