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Previous studies reported that cells are exposed to and react with free\u2010radicals produced during encapsulation, creating a pericellular region of reduced crosslink density measured by the distance R\n                    <jats:sub>d<\/jats:sub>\n                    . This study tests the hypothesis that increasing R\n                    <jats:sub>d<\/jats:sub>\n                    improves spatial elaboration of deposited extracellular matrix (ECM) (i.e., micro\u2010tissue) in a degrading hydrogel. Chondrocytes pre\u2010treated or not with the antioxidant ascorbic acid atlow (1\u00a0nM) and high (1000\u00a0nM) concentrations prior to encapsulation resulted in increasingly larger R\n                    <jats:sub>d<\/jats:sub>\n                    \u2019s: 3.3 (0\u00a0nM), 6.7 (1\u00a0nM), and 10 (1000\u00a0nM) \u00b5m. Encapsulated chondrocytes when cultured up to ten weeks, produced micro\u2010tissues within the hydrogels. The median micro\u2010tissue area increased by 29% (1\u00a0nM) and 570% (1000\u00a0nM) compared to 0\u00a0nM condition. This work shows that bolstering antioxidant defense mechanisms enhances chondrocyte inhibition of the polymerization immediately around the cell, resulting in larger regions of reduced crosslinking. This local variation in network structure, which degrades more quickly, led to improved neotissue assembly and larger micro\u2010tissues, comprised primarily of sulfated glycosaminoglycans and collagen type II. This approach offers a novel way to improve ECM elaboration and interconnectivity in free\u2010radical polymerized hydrogels for tissue engineering.\n                  <\/jats:p>","DOI":"10.1002\/mabi.202300481","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T04:29:32Z","timestamp":1783571372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antioxidant Ascorbic Acid Influences Cell\u2010mediated Alterations in Localized Hydrogel Crosslinking and Affects Neo\u2010Cartilage Connectivity in Hydrolytically Degradable Hydrogels"],"prefix":"10.1002","volume":"26","author":[{"given":"Mollie M.","family":"Maples","sequence":"first","affiliation":[{"name":"Department of Chemical and Biological Engineering University of Colorado  Boulder Colorado 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