{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T03:20:04Z","timestamp":1773804004282,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T00:00:00Z","timestamp":1711670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013465","name":"French Muscular Dystrophy Association","doi-asserted-by":"publisher","award":["20609"],"award-info":[{"award-number":["20609"]}],"id":[{"id":"10.13039\/100013465","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["17\u2010CE18\u20100015\u201001\u2010VINP"],"award-info":[{"award-number":["17\u2010CE18\u20100015\u201001\u2010VINP"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Three different amphiphilic block copolymer families are synthesized to investigate new opportunities to enhance gene delivery via Hydrodynamic Limb Vein (HLV) injections. First a polyoxazoline\u2010based family containing mostly one poly(2\u2010methyl\u20102\u2010oxazoline) (PMeOx) block and a second block POx with an ethyl (EtOx), isopropyl (iPrOx) or phenyl substituent (PhOx) is synthesized. Then an ABC poly(2\u2010ethyl\u20102\u2010oxazoline)\u2010<jats:italic>b<\/jats:italic>\u2010poly(2\u2010n\u2010propyl\u20102\u2010oxazoline)\u2010<jats:italic>b\u2010<\/jats:italic>poly(2\u2010methyl\u20102\u2010oxazoline) triblock copolymer is synthesized, with a thermosensitive middle block. Finally, polyglycidol\u2010<jats:italic>b<\/jats:italic>\u2010polybutylenoxide\u2010<jats:italic>b<\/jats:italic>\u2010polyglycidol copolymers with various molar masses and amphiphilic balance are produced. The simple architecture of neutral amphiphilic triblock copolymer is not sufficient to obtain enhanced in vivo gene transfection. Double or triple amphiphilic neutral block copolymers are improving the in vivo transfection performances through HLV administration as far as a block having an lower critical solution temperature is incorporated in the vector. The molar mass of the copolymer does not seem to affect the vector performances in a significant manner.<\/jats:p>","DOI":"10.1002\/mabi.202300568","type":"journal-article","created":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T15:55:15Z","timestamp":1711036515000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Neutral Block Copolymer Assisted Gene Delivery using Hydrodynamic Limb Vein Injection"],"prefix":"10.1002","volume":"24","author":[{"given":"Yann Le","family":"Guen","sequence":"first","affiliation":[{"name":"Univ Brest INSERM EFS  UMR 1078 GGB\u2010GTCA Team Brest F\u201029200 France"},{"name":"CHU de Brest Service de G\u00e9n\u00e9tique M\u00e9dicale et de Biologie de la Reproduction Centre de R\u00e9f\u00e9rence des Maladies Rares Maladies Neuromusculaires  Brest 29200 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gwendoline","family":"Delecourt","sequence":"additional","affiliation":[{"name":"Institut Parisien de Chimie Mol\u00e9culaire Equipe Chimie des Polym\u00e8res Sorbonne University UMR 8232 CNRS Paris 75005 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tony Le","family":"Gall","sequence":"additional","affiliation":[{"name":"Univ Brest INSERM EFS  UMR 1078 GGB\u2010GTCA Team Brest F\u201029200 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiqin","family":"Du","sequence":"additional","affiliation":[{"name":"Institut Parisien de Chimie Mol\u00e9culaire Equipe Chimie des Polym\u00e8res Sorbonne University UMR 8232 CNRS Paris 75005 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Illy","sequence":"additional","affiliation":[{"name":"Institut Parisien de Chimie Mol\u00e9culaire Equipe Chimie des Polym\u00e8res Sorbonne University UMR 8232 CNRS Paris 75005 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9cile","family":"Huin","sequence":"additional","affiliation":[{"name":"Institut Parisien de Chimie Mol\u00e9culaire Equipe Chimie des Polym\u00e8res Sorbonne University UMR 8232 CNRS Paris 75005 France"},{"name":"University of Evry  Essonne Evry 91000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00e9ronique","family":"Bennevault","sequence":"additional","affiliation":[{"name":"Institut Parisien de Chimie Mol\u00e9culaire Equipe Chimie des Polym\u00e8res Sorbonne University UMR 8232 CNRS Paris 75005 France"},{"name":"University of Evry  Essonne Evry 91000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Midoux","sequence":"additional","affiliation":[{"name":"Centre de Biophysique Mol\u00e9culaire  CNRS UPR4301 Orl\u00e9ans 45100 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tristan","family":"Montier","sequence":"additional","affiliation":[{"name":"Univ Brest INSERM EFS  UMR 1078 GGB\u2010GTCA Team Brest F\u201029200 France"},{"name":"CHU de Brest Service de G\u00e9n\u00e9tique M\u00e9dicale et de Biologie de la Reproduction Centre de R\u00e9f\u00e9rence des Maladies Rares Maladies Neuromusculaires  Brest 29200 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4919-0779","authenticated-orcid":false,"given":"Philippe","family":"Gu\u00e9gan","sequence":"additional","affiliation":[{"name":"Institut Parisien de Chimie Mol\u00e9culaire Equipe Chimie des Polym\u00e8res Sorbonne University UMR 8232 CNRS Paris 75005 France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,29]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2022483"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/v11010028"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/PHM.0000000000000138"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad5143"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-2254(199907\/08)1:4<245::AID-JGM49>3.0.CO;2-G"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2006.186"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2009.12.014"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1126\/science.1690918","volume":"247","author":"Wolff J. A.","journal-title":"Science"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2004.09.011"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms16105"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.21.7413"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.3150"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007548826495"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym11040745"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.1304"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3301189"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2005.16.1318"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp0687302"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.201900276"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2006.11.025"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-008-9698-9"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eurpolymj.2016.09.042"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200902672"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm5016656"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/masy.200850122"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.0c00775"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm5002608"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.7b00158"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.5b01456"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.2c21683"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2022.103"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-26714-w"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/pola.24417"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsapm.0c00308"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.9b03687"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(97)00215-0"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eurpolymj.2019.109323"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/macp.201900210"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq922"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.1612"}],"container-title":["Macromolecular Bioscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mabi.202300568","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T13:12:53Z","timestamp":1721049173000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mabi.202300568"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,29]]},"references-count":40,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1002\/mabi.202300568"],"URL":"https:\/\/doi.org\/10.1002\/mabi.202300568","archive":["Portico"],"relation":{},"ISSN":["1616-5187","1616-5195"],"issn-type":[{"value":"1616-5187","type":"print"},{"value":"1616-5195","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,29]]},"assertion":[{"value":"2023-12-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2300568"}}