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Decellularized extracellular matrix (dECM) hydrogel is a popular biomaterial choice for tissue engineering and studying carcinogenesis as a tumor microenvironmental mimetic. This study proposes a method for high throughput bioprinting with decellularized adipose tissue (DAT) based hydrogels for 3D breast cancer modeling. A comparative analysis of decellularization protocol using detergent\u2010based and detergent\u2010free decellularization methods for caprine\u2010origin adipose tissue is performed, and the efficacy of dECM hydrogel for 3D cancer modeling is assessed. Histological, biochemical, morphological, and biological characterization and analysis showcase the cytocompatibility of DAT hydrogel. The rheological property of DAT hydrogel and printing process optimization is assessed to select a bioprinting window to attain 3D breast cancer models. The bioprinted tissues are characterized for cellular viability and tumor cell\u2010matrix interactions. Additionally, an approach for breast cancer modeling is shown by performing rapid high throughput bioprinting in a 96\u2010well plate format, and in vitro drug screening using 5\u2010fluorouracil is performed on 3D bioprinted microtumors. The results of this study suggest that high throughput bioprinting of cancer models can potentially have downstream clinical applications like multi\u2010drug screening platforms and personalized disease models.<\/jats:p>","DOI":"10.1002\/mabi.202400035","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T06:29:34Z","timestamp":1714458574000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["High Throughput Bioprinting Using Decellularized Adipose Tissue\u2010Based Hydrogels for 3D Breast Cancer Modeling"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5213-3794","authenticated-orcid":false,"given":"Priyanshu","family":"Shukla","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Indian Institute of Technology Hyderabad  Kandi Sangareddy Telangana 502284 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashis Kumar","family":"Bera","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Indian Institute of Technology Hyderabad  Kandi Sangareddy Telangana 502284 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sriya","family":"Yeleswarapu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Indian Institute of Technology Hyderabad  Kandi Sangareddy Telangana 502284 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3588-1800","authenticated-orcid":false,"given":"Falguni","family":"Pati","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Indian Institute of Technology Hyderabad  Kandi Sangareddy Telangana 502284 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,29]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2958"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201805510"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2019.100008"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19103028"},{"key":"e_1_2_9_5_1","volume":"1","author":"Gilpin A.","year":"2017","journal-title":"Biomed. 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