{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T14:48:34Z","timestamp":1778510914152,"version":"3.51.4"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:00:00Z","timestamp":1718668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100015019","name":"Chongqing University of Science and Technology","doi-asserted-by":"publisher","award":["YKJCX2120514"],"award-info":[{"award-number":["YKJCX2120514"]}],"id":[{"id":"10.13039\/501100015019","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A cryogel is a supermacroporous gel network that is generated at subzero temperatures by polymerizing monomers or gelating polymeric precursors. Since cryogels possess inherent characteristics such as\u00a0interconnected\u00a0macroporous structures, excellent mechanical properties,\u00a0and high resistance\u00a0to autoclave sterilization,\u00a0they are highly desirable for tissue engineering and regenerative medicine. Silk fibroin, a natural protein obtained\u00a0from <jats:italic>Bombyx mori<\/jats:italic>\u00a0silkworms, is an excellent raw material for cryogel preparation. The aim of this study is to\u00a0establish a\u00a0controlled method for preparing silk fibroin\u00a0cryogels with suitable properties for application as tissue engineering scaffolds. Using a dual crosslinking strategy consisting of low\u2010temperature radical polymerization coupled with methanol\u2010induced conformational transformation, porous cryogels are prepared. The cryogels\u00a0display many unique characteristics, such as an interconnected macroporous structure, a high water absorption capacity, water\u2010triggered shape memory, syringe injectability, and strong resilience to autoclave sterilization. Furthermore, the cryogels demonstrate excellent biocompatibility and cell affinity, facilitating cell adhesion, migration, and\u00a0proliferation. The interconnected supermacroporous architecture resembling the native\u00a0extracellular matrix, together with their unique physical properties and autoclaving stability, suggests that cryogels are promising candidate scaffolds for tissue engineering and cell therapy.<\/jats:p>","DOI":"10.1002\/mabi.202400038","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T18:30:00Z","timestamp":1717698600000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Preparation of Autoclavable and Injectable Silk Fibroin Cryogels for Tissue Engineering Applications"],"prefix":"10.1002","volume":"24","author":[{"given":"Hongjuan","family":"Han","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Nano\/Micro Composite Materials and Devices, School of Metallurgy and Materials Engineering Chongqing University of Science and Technology  Chongqing 401331 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyan","family":"Li","sequence":"additional","affiliation":[{"name":"Chongqing Engineering Laboratory of Nano\/Micro Biomedical Detection Technology, School of Metallurgy and Materials Engineering Chongqing University of Science and Technology  Chongqing 401331 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Nano\/Micro Composite Materials and Devices, School of Metallurgy and Materials Engineering Chongqing University of Science and Technology  Chongqing 401331 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Nano\/Micro Composite Materials and Devices, School of Metallurgy and Materials Engineering Chongqing University of Science and Technology  Chongqing 401331 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoqing","family":"Guan","sequence":"additional","affiliation":[{"name":"Chongqing Engineering Laboratory of Nano\/Micro Biomedical Detection Technology, School of Metallurgy and Materials Engineering Chongqing University of Science and Technology  Chongqing 401331 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingzhi","family":"Yao","sequence":"additional","affiliation":[{"name":"Chongqing Engineering Laboratory of Nano\/Micro Biomedical Detection Technology, School of Metallurgy and Materials Engineering Chongqing University of Science and Technology  Chongqing 401331 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8445-6125","authenticated-orcid":false,"given":"Wenqian","family":"Xiao","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Nano\/Micro Composite Materials and Devices, School of Metallurgy and Materials Engineering Chongqing University of Science and Technology  Chongqing 401331 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Li","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Nano\/Micro Composite Materials and Devices, School of Metallurgy and Materials Engineering Chongqing University of Science and Technology  Chongqing 401331 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoling","family":"Liao","sequence":"additional","affiliation":[{"name":"Chongqing Engineering Laboratory of Nano\/Micro Biomedical Detection Technology, School of Metallurgy and Materials Engineering Chongqing University of Science and Technology  Chongqing 401331 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,6,18]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat1421"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr000108x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(03)00340-5"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8556"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.0c00923"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/gels4030077"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2003.08.002"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-008-3504-4"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2017.08.033"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-05846-7_3"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2019.09.008"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201900679"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/mame.201900824"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2012.09.043"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm3018033"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.09.087"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym12092105"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201503450"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.7b04623"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202203050"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.202200189"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03759-y"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/ma0610109"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2014.10.027"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.36777"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.201500013"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.120281"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-021-00622-1"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfa.2024.134060"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9RA09239K"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm050431f"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm050622i"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.2c12503"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201500898"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04998-9"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2023.120545"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2014.05.059"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2017.10.015"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.3c09337"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2017.11.005"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.201300321"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201601066"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.11.044"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.202100234"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.091"}],"container-title":["Macromolecular Bioscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mabi.202400038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T13:33:25Z","timestamp":1729690405000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mabi.202400038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,18]]},"references-count":45,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1002\/mabi.202400038"],"URL":"https:\/\/doi.org\/10.1002\/mabi.202400038","archive":["Portico"],"relation":{},"ISSN":["1616-5187","1616-5195"],"issn-type":[{"value":"1616-5187","type":"print"},{"value":"1616-5195","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,18]]},"assertion":[{"value":"2024-01-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2400038"}}