{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T12:36:54Z","timestamp":1788093414256,"version":"build-2803163510"},"reference-count":70,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T00:00:00Z","timestamp":1720569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["#22K15461"],"award-info":[{"award-number":["#22K15461"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008732","name":"Uehara Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008732","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021711","name":"Bayerisches Staatsministerium f\u00fcr Wissenschaft und Kunst","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["326998133"],"award-info":[{"award-number":["326998133"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["270563345"],"award-info":[{"award-number":["270563345"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["210879364"],"award-info":[{"award-number":["210879364"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The ubiquitous mold <jats:italic>Aspergillus fumigatus<\/jats:italic> (<jats:italic>A. fumigatus<\/jats:italic>) is one of the main fungal pathogens causing invasive infections in immunocompromised humans. Conventional antifungal agents exhibit limited efficacy and often cause severe side effects. Nanoparticle\u2010based antifungal delivery provides a promising alternative, which can increase local drug concentration; while, mitigating toxicity, thereby enhancing treatment efficacy. Previous research underscores the potential of poly(glycidol)\u2010based nanogels (NG) with negative surface charge as carriers for delivering antifungals to <jats:italic>A. fumigatus<\/jats:italic> hyphae. In this study, NG is tailored with 2\u2010carboxyethyl acrylate (CEA) or with phosphoric acid 2\u2010hydroxyethyl acrylate (PHA). It is discovered that quenching with PHA clearly improves the adhesion of NG to hyphal surface and the internalization of NG into the hyphae under protein\u2010rich conditions, surpassing the outcomes of non\u2010quenched and CEA\u2010quenched NG. This enhancement cannot be solely attributed to an increase in negative surface charge but appears to be contingent on the functional group of the quencher. Further, it is demonstrated that itraconazole\u2010loaded, PHA\u2010functionalized nanogels (NGxPHA\u2010ITZ) show lower MIC in vitro and superior therapeutic effect in vivo against <jats:italic>A. fumigatus<\/jats:italic> compared to pure itraconazole. This confirms NGxPHA as a promising antifungal delivery system.<\/jats:p>","DOI":"10.1002\/mabi.202400082","type":"journal-article","created":{"date-parts":[[2024,6,8]],"date-time":"2024-06-08T08:53:44Z","timestamp":1717836824000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Beyond the Charge: Interplay of Nanogels\u2019 Functional Group and Zeta\u2010Potential for Antifungal Drug Delivery to Human Pathogenic Fungus <i>Aspergillus Fumigatus<\/i>"],"prefix":"10.1002","volume":"24","author":[{"given":"Theresa","family":"Vogel","sequence":"first","affiliation":[{"name":"Department for Functional Materials in Medicine and Dentistry Institute of Functional Materials and Biofabrication University of W\u00fcrzburg  97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Kohlmann","sequence":"additional","affiliation":[{"name":"Department for Functional Materials in Medicine and Dentistry Institute of Functional Materials and Biofabrication University of W\u00fcrzburg  97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahraa","family":"Abboud","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II Center for Experimental Molecular Medicine W\u00fcrzburg University Hospital  97078 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sina","family":"Thusek","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II Center for Experimental Molecular Medicine W\u00fcrzburg University Hospital  97078 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"Fella","sequence":"additional","affiliation":[{"name":"Department for Functional Materials in Medicine and Dentistry Institute of Functional Materials and Biofabrication University of W\u00fcrzburg  97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7057-5369","authenticated-orcid":false,"given":"Joerg","family":"Te\u00dfmar","sequence":"additional","affiliation":[{"name":"Department for Functional Materials in Medicine and Dentistry Institute of Functional Materials and Biofabrication University of W\u00fcrzburg  97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhisa","family":"Sekimizu","sequence":"additional","affiliation":[{"name":"Endowed Course \u201cDrug Discoveries by Silkworm Models,\u201d Faculty of Pharmaceutical Sciences Teikyo University Tokyo 192\u20100395 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Miyashita","sequence":"additional","affiliation":[{"name":"Institute of Medical Mycology Teikyo University  Tokyo 192\u20100395 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3165-2648","authenticated-orcid":false,"given":"Andreas","family":"Beilhack","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II Center for Experimental Molecular Medicine W\u00fcrzburg University Hospital  97078 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3167-8466","authenticated-orcid":false,"given":"J\u00fcrgen","family":"Groll","sequence":"additional","affiliation":[{"name":"Department for Functional Materials in Medicine and Dentistry Institute of Functional Materials and Biofabrication University of W\u00fcrzburg  97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2845-1175","authenticated-orcid":false,"given":"Yidong","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II Center for Experimental Molecular Medicine W\u00fcrzburg University Hospital  97078 W\u00fcrzburg Germany"},{"name":"JSPS International Research Fellow Endowed Course \u201cDrug Discoveries by Silkworm Models,\u201d Faculty of Pharmaceutical Sciences Teikyo University Tokyo 192\u20100395 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2285-2955","authenticated-orcid":false,"given":"Krystyna","family":"Albrecht","sequence":"additional","affiliation":[{"name":"Department for Functional Materials in Medicine and Dentistry Institute of Functional Materials and Biofabrication University of W\u00fcrzburg  97070 W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,10]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40588-022-00181-3"},{"key":"e_1_2_8_2_1","volume":"65","author":"Fernandes C. M.","year":"2021","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa6097"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-022-00720-1"},{"key":"e_1_2_8_5_1","unstructured":"World Health Organization WHO Fungal Priority Pathogens List to Guide Research Development and Public Health Action2022 https:\/\/www.who.int\/publications\/i\/item\/9789240060241."},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.2.310"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.12367"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(01)02104-7"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00014-09"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13213"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo10030106"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/13543784.2020.1791819"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-018-3251-5"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2020.e04663"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjm-2017-0749"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2021.09.019"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4CS00341A"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.26502\/jppr.0001"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.0c00489"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/anbr.202000060"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.8b03241"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.4161\/rna.6.2.7717"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7NR04724J"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00373"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-016-1633-3"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.201200389"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/pola.23595"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/cm402180t"},{"key":"e_1_2_8_29_1","volume-title":"Handbook of Chemistry and Physics","author":"Lide D.","year":"1986"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-48510-1"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01379a005"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.39.6.853"},{"key":"e_1_2_8_33_1","first-page":"169183","volume-title":"Aspergillus fumigatus Aspergillosis","author":"Mouyna I.","year":"2008"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.00028"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.FUNK\u20100035\u20102016"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm700701w"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc800547c"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar200216f"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1081\/DRT-200047900"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/cplu.202000496"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.8b01553"},{"key":"e_1_2_8_42_1","first-page":"95","volume":"3","author":"Sultana F.","year":"2013","journal-title":"J. Appl. Pharm. Sci."},{"key":"e_1_2_8_43_1","unstructured":"EUCAST Method for Susceptibility Testing of Moulds https:\/\/www.eucast.org\/astoffungi\/methodsinantifungalsusceptibilitytesting\/ast_of_moulds(accessed: April 2024)."},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph13090248"},{"key":"e_1_2_8_45_1","unstructured":"Merck Protocol Guide WST\u20101 Assay for Cell Proliferation and Viability https:\/\/www.sigmaaldrich.com\/DE\/de\/technical\u2010documents\/protocol\/cell\u2010culture\u2010and\u2010cell\u2010culture\u2010analysis\/cell\u2010counting\u2010and\u2010health\u2010analysis\/cell\u2010proliferation\u2010reagent\u2010wst\u20101(accessed: April 2024)."},{"key":"e_1_2_8_46_1","unstructured":"Biological evaluation of medical devices \u2013 Part 5: Tests for in vitro cytotoxicity (ISO 10993\u20135:2009)."},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2015.06.006"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym12112576"},{"key":"e_1_2_8_49_1","unstructured":"ATCC TIB\u2010202.https:\/\/www.atcc.org\/products\/tib\u2010202(accessed April 2024)."},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-023-03574-7"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.5b01226"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics11120974"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-022-03301-2"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt075"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/ccr3.3726"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/ja.2016.106"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.3314\/jjmm.38.175"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7020136"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.35.10.2053"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-46013-1"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.MB\u20100017\u20102015"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201206266"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05003-14"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2cs15327k"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12253"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0259-1_14"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.459222"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.133.3465.1704"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.4172\/2161-1459.1000189"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12668"}],"container-title":["Macromolecular Bioscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mabi.202400082","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T13:34:43Z","timestamp":1729690483000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mabi.202400082"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,10]]},"references-count":70,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1002\/mabi.202400082"],"URL":"https:\/\/doi.org\/10.1002\/mabi.202400082","archive":["Portico"],"relation":{},"ISSN":["1616-5187","1616-5195"],"issn-type":[{"value":"1616-5187","type":"print"},{"value":"1616-5195","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,10]]},"assertion":[{"value":"2024-02-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2400082"}}