{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T22:10:52Z","timestamp":1789683052858,"version":"4.0.1"},"reference-count":58,"publisher":"Wiley","license":[{"start":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T00:00:00Z","timestamp":1722643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The complex anatomy of the cornea and the subsequent keratocyte\u2010fibroblast transition have always made corneal stromal regeneration difficult. Recently, 3D printing has received considerable attention\u00a0in terms of fabrication of scaffolds\u00a0with precise dimension and pattern. In the current work, 3D printable polymer hydrogels made of GelMA\/agarose are formulated and its rheological properties are evaluated. Despite the variation in agarose content, both the hydrogels exhibited G\u2032&gt;G\u2032\u2032 modulus. A prototype for 3D stromal model is created using Solid Works software, mimicking the anatomy of an adult cornea. The fabrication of 3D\u2010printed hydrogels is performed using pneumatic extrusion. The FTIR analysis speculated that the hydrogel is well crosslinked and established strong hydrogen bonding with each other, thus contributing to improved thermal and structural stability. The MTT analysis revealed a higher rate of cell proliferation on the hydrogels. The optical analysis carried out on the 14th day of incubation revealed that the hydrogels exhibit transparency matching with natural corneal stromal tissue. Specific protein marker expression confirmed the keratocyte phenotype and showed that the cells do not undergo terminal differentiation into stromal fibroblasts. The findings of this work point to the potential of GelMA\/A hydrogels as a novel biomaterial for corneal stromal tissue engineering.<\/jats:p>","DOI":"10.1002\/mabi.202400136","type":"journal-article","created":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T06:33:59Z","timestamp":1722666839000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Fabrication of GelMA \u2013 Agarose Based 3D Bioprinted Photocurable Hydrogel with In Vitro Cytocompatibility and Cells Mirroring Natural Keratocytes for Corneal Stromal Regeneration"],"prefix":"10.1002","author":[{"given":"Renuka","family":"Vijayaraghavan","sequence":"first","affiliation":[{"name":"Electrochemical Process Engineering CSIR\u2010Central Electrochemical Research Institute (CECRI)  Karaikudi Tamil Nadu 630003 India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5113-4672","authenticated-orcid":false,"given":"Sravanthi","family":"Loganathan","sequence":"additional","affiliation":[{"name":"Electrochemical Process Engineering CSIR\u2010Central Electrochemical Research Institute (CECRI)  Karaikudi Tamil Nadu 630003 India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi Babu","family":"Valapa","sequence":"additional","affiliation":[{"name":"Electrochemical Process Engineering CSIR\u2010Central Electrochemical Research Institute (CECRI)  Karaikudi Tamil Nadu 630003 India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,3]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1038\/s41598-017-01072-0","volume":"7","author":"Kong B.","year":"2017","journal-title":"Sci. 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