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In this study, the previously developed streptavidin platforms is aimed to use to investigate the role of glycosaminoglycans (GAGs) in bone morphogenetic protein 2 (BMP2) signaling. However, it is observed that the interpretation of findings is skewed due to the GAG\u2010unrelated, non\u2010specific binding of BMP2 on components of biomaterials. Non\u2010specific adsorption of proteins is a recurrent and challenging issue for biomaterial studies. Despite the initial incorporation of anti\u2010fouling polyethylene glycol (PEG) chains within biomaterials, the residual non\u2010specific BMP2 adsorption still triggered BMP2 signaling within the same range as conditions of interest. The various options are explored to prevent BMP2 non\u2010specific adsorption and a successful blocking condition involving a combination of bovine serum albumin and trehalose are identified. Furthermore, the effect of this blocking step improved when using gold platforms instead of glass, particularly with Chinese hamster ovary (CHO) cells. With this specific example, it is suggested that non\u2010specific adsorption of BMPs on biomaterials may be a general concern \u2013 often undetected by classical surface\u2010sensitive techniques \u2013 that needs to be addressed to better interpret cellular responses.<\/jats:p>","DOI":"10.1002\/mabi.202400169","type":"journal-article","created":{"date-parts":[[2024,8,31]],"date-time":"2024-08-31T09:34:22Z","timestamp":1725096862000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["BMP2 Binds Non\u2010Specifically to PEG\u2010Passivated Biomaterials and Induces pSMAD 1\/5\/9 Signalling"],"prefix":"10.1002","volume":"24","author":[{"given":"Jean Le","family":"Pennec","sequence":"first","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, INSERM, CEA, CNRS  U1292 Biosant\u00e9, EMR 5000 Grenoble 38000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amaury","family":"Guibert","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, INSERM, CEA, CNRS  U1292 Biosant\u00e9, EMR 5000 Grenoble 38000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raviteja","family":"Gurram","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, INSERM, CEA, CNRS  U1292 Biosant\u00e9, EMR 5000 Grenoble 38000 France"},{"name":"Universit\u00e9 Grenoble Alpes, CNRS, LIPhy  Grenoble 38000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antoine","family":"Delon","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, CNRS, LIPhy  Grenoble 38000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romain R.","family":"Viv\u00e8s","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, CNRS, CEA, IBS  Grenoble 38000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3264-2027","authenticated-orcid":false,"given":"Elisa","family":"Migliorini","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, INSERM, CEA, CNRS  U1292 Biosant\u00e9, EMR 5000 Grenoble 38000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,31]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surfcoat.2013.04.028"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/s19112488"},{"key":"e_1_2_9_3_1","first-page":"1","volume-title":"Protein Adsorption and Materials Biocompatibility: A Tutorial Review and Suggested Hypotheses","author":"Andrade J. 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