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In this composite, BMP\u20102 is immobilized on microparticles of diopside while lysostaphin is mixed directly into the hydrogel, providing sustained release of BMP\u20102 to allow gradual bone formation and rapid release of lysostaphin to eliminate infection immediately after implantation. Introduction of diopside of up to 3% (w\/v) has a negligible effect on the mechanical properties of the hydrogel but provides a high sorption capacity for BMP\u20102. The hydrogels show good biocompatibility and antibacterial activity. Lysostaphin released from the implants over a 3\u00a0h period efficiently kills planktonic cells and completely destroys 24\u00a0h pre\u2010formed biofilms of <jats:italic>Staphylococcus aureus<\/jats:italic>. Furthermore, in vivo experiments in a mouse model of critically\u2010sized cranial defects infected with <jats:italic>S. aureus<\/jats:italic> show a complete lack of osteogenesis when implants contain only BMP\u20102, whereas, in the presence of lysostaphin, complete closure of the defect with newly formed mineralized bone tissue is observed. Thus, the new implantable gellan\u2010xanthan hydrogel with diopside and recombinant lysostaphin and BMP\u20102 shows both osteogenic and antibacterial properties and represents a promising material for the treatment and\/or prevention of osteomyelitis after bone trauma.<\/jats:p>","DOI":"10.1002\/mabi.202400205","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T11:45:00Z","timestamp":1723635900000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Dual\u2010Functional Implant Based on Gellan\u2010Xanthan Hydrogel with Diopside, BMP\u20102 and Lysostaphin for Bone Defect Repair and Control of Staphylococcal Infection"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2580-3489","authenticated-orcid":false,"given":"Anna S.","family":"Karyagina","sequence":"first","affiliation":[{"name":"Gamaleya National Research Center for Epidemiology and 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