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Incorporation of these extracellular\u2010matrix (ECM)\u2010derived molecules into a hydrogel model can mimic in vivo conditions to enable dentin\u2013pulp complex regeneration. Here, a chitosan\/alginate (C\/A) hydrogel is developed to sequester bovine ECM extracts. Human dental pulp cells (hDPCs) are cultured with these constructs and proliferation and cytotoxicity assays confirm that these C\/A hydrogels are bioactive. Sequential <jats:italic>z<\/jats:italic>\u2010axis fluorescent imaging visualizes hDPCs protruding into the hydrogel as it degraded. Alizarin red S staining shows that hDPCs cultured with the hydrogels display increased calcium\u2010ion deposition, with dentin ECM stimulating the highest levels. Alkaline phosphatase activity is increased, as is expression of transforming growth factor\u2010beta as demonstrated using immunocytochemistry. Directional analysis following phase contrast kinetic image capture demonstrates that both dentin and pulp ECM molecules act as chemoattractants for hDPCs. Data from this study demonstrate that purified ECM from dental pulp and dentin when delivered in a C\/A hydrogel stimulates dental tissue repair processes in vitro.<\/jats:p>","DOI":"10.1002\/mabi.202400254","type":"journal-article","created":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T06:38:13Z","timestamp":1719556693000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Harnessing the Regenerative Potential of Purified Bovine Dental Pulp and Dentin Extracellular Matrices in a Chitosan\/Alginate Hydrogel"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3733-1359","authenticated-orcid":false,"given":"Maree L.","family":"Gould","sequence":"first","affiliation":[{"name":"Faculty of Dentistry Sir John Walsh Research Institute University of Otago  P.O. 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