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First Halomonas levan (HL) polysaccharide is hydrolyzed (hHL) and subjected to sulfation to attain Sulfated hydrolyzed Halomonas levan (ShHL)\u2010based material indicating heparin mimetic properties. Then, optimization studies are carried out to produce coaxially generated radially aligned Poly(caprolactone) (PCL) \u2010ShHL nanofibers via electrospinning. The obtained nanofibers are characterized with Fourier Transform Infrared Spectroscopy (FTIR)\u00a0and Field Emission Scanning Electron Microscopy with Energy Dispersive X\u2010Ray (FESEM\u2010EDX) analysis, and mechanical, contact angle measurement, biodegradability, and swelling tests as well. Afterward, cytotoxicity of artificial tympanic membranes is analyzed by MTT (3\u2010(4,5\u2010Dimethylthiazol\u20102\u2010yl) \u20102,5 Diphenyltetrazolium Bromide) test, and their impacts on cell proliferation, cellular adhesion, wound healing processes are explored. Furthermore, an additional FESEM imaging is performed to manifest the interactions between fibroblasts and nanofibers. According to analytical measurements it is detected that PCL\u2010ShHL nanofibers i) are smaller in fiber diameter, ii) are more biodegradable, iii) are more hydrophilic, and iv) demonstrated superior mechanical properties compared to PCL nanofibers. Moreover, it is also deciphered that PCL\u2010ShHL nanofibers strongly elevated cellular adhesion, proliferation, and in vitro wound healing features compared to PCL nanofibers. According to obtained results it is assumed that newly synthetized levan and PCL mediated nanofibers are very encouraging for healing tympanic membrane perforations.<\/jats:p>","DOI":"10.1002\/mabi.202400291","type":"journal-article","created":{"date-parts":[[2024,10,27]],"date-time":"2024-10-27T02:10:11Z","timestamp":1729995011000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Manufacturing Radially Aligned PCL Nanofibers Reinforced With Sulfated Levan and Evaluation of its Biological Activity for Healing Tympanic Membrane Perforations"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3566-8874","authenticated-orcid":false,"given":"Busra","family":"Akgul","sequence":"first","affiliation":[{"name":"Department of Bioengineering Yildiz Technical University  Istanbul 34220 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cansu","family":"Gulcan","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Yildiz Technical University  Istanbul 34220 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selay","family":"Tornaci","sequence":"additional","affiliation":[{"name":"IBSB Department of Bioengineering Marmara University  Istanbul 34854 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merve","family":"Erginer","sequence":"additional","affiliation":[{"name":"Institute of Nanotechnology and Biotechnology Istanbul University\u2010Cerrahpa\u015fa  Istanbul 34500 Turkey"},{"name":"Health Biotechnology Joint Research and Application Center of Excellence  Esenler Istanbul 34220 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebru","family":"Toksoy Oner","sequence":"additional","affiliation":[{"name":"IBSB Department of Bioengineering Marmara University  Istanbul 34854 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emrah Sefik","family":"Abamor","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Yildiz Technical University  Istanbul 34220 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6662-6642","authenticated-orcid":false,"given":"Serap","family":"Acar","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Yildiz Technical University  Istanbul 34220 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adil M.","family":"Allahverdiyev","sequence":"additional","affiliation":[{"name":"The V. 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