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Photocrosslinkable hydrogels, such as gelatin methacrylate (GelMA), have gained attention for their unique properties but often lack antimicrobial activity. To enhance their effectiveness, researchers are exploring methods to incorporate antimicrobial agents into photocrosslinkable hydrogel dressings. Immobilization of antimicrobial peptides (AMPs) onto hydrogel matrices may be achieved through physical or chemical methods. Although, chemical immobilization, using techniques like EDC\/NHS chemistry, has shown promise in enhancing antimicrobial properties of hydrogels, the capacity for immobilization may be limited by the structure of hydrogel. Physical methods, such as immersing, offer alternatives but may have different efficacy and biocompatibility. The study aims to chemically immobilize GelMA with P9\u20104 AMP by photoinduced conjugation and EDC\/NHS chemistry and compare its antimicrobial efficacy with a physical immobilization method. Chemical immobilization by EDC\/NHS chemistry significantly enhances the antimicrobial effect of GelMA hydrogels against multi\u2010drug resistant <jats:italic>Psuedomonas aeruginosa<\/jats:italic> (MDR <jats:italic>P. aeruginosa<\/jats:italic>) and methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA) while maintaining favorable biocompatibility. Study highlights the potential of AMP\u2010functionalized GelMA as advanced wound dressings for reducing infections caused by antibiotic\u2010resistant bacteria and offers a promising approach for future research in wound management.<\/jats:p>","DOI":"10.1002\/mabi.202400324","type":"journal-article","created":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T13:31:45Z","timestamp":1725456705000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The Effect of Immobilization Methods of P9\u20104 Antimicrobial Peptide Onto Gelatin Methacrylate on Multidrug\u2010Resistant Bacteria: A Comparative Study"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0895-4768","authenticated-orcid":false,"given":"G\u00fcnnur","family":"Pulat","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering \u0130zmir Katip \u00c7elebi University  \u0130zmir 35620 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisa Nilsu","family":"\u00c7elebi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering \u0130zmir Katip \u00c7elebi University  \u0130zmir 35620 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eda","family":"Bilgi\u00e7","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering \u0130zmir Katip \u00c7elebi University  \u0130zmir 35620 Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,4]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.2165\/00128071-200001050-00002"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.2.244-269.2001"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.02.002"},{"key":"e_1_2_8_4_1","first-page":"3","volume-title":"Methods in Molecular Biology","author":"Haney E. 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