{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T18:08:41Z","timestamp":1789668521932,"version":"4.0.1"},"reference-count":79,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T00:00:00Z","timestamp":1728864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In response to the critical issue of chronic wound management, this research explores the development of a multiple\u2010layer biomaterial loaded with LTX\u2010109 a novel broad\u2010spectrum topical antimicrobial peptide currently investigated for the treatment of bacterial skin infections. The novel patch is conceived to load and preserve the function of LTX\u2010109, release it on site in a progressive manner, and therefore make available a device for simultaneous wounds disinfection and tissues healing. Chitosan, tannic acid and glycerol along with the solvent casting process are selected for the development of a multilayer structure in which each single layer is designed by choosing a specific composition and stability to tune its behavior and function. On the top, a protective layer to protect the wound from external contaminations, in the middle a medicated layer loaded with LTX\u2010109 and at the bottom a multifunctional layer to modulate the release of LTX\u2010109. Extensive characterizations show that the patch meets the essential requirements for creating an effective wound healing environment, such as absorption of exudate, maintenance of good oxygen and moisture permeability, biodegradability, biocompatibility, and sustained release of LTX\u2010109 with fully retained antibacterial activity as demonstrated by MIC values obtained against reference bacteria.<\/jats:p>","DOI":"10.1002\/mabi.202400375","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T18:08:33Z","timestamp":1728929313000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Multiple\u2010Layer Chitosan\u2010Based Patches Medicated With LTX\u2010109 Antimicrobial Peptide for Modulated Local Therapy in the Management of Chronic Wounds"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0214-103X","authenticated-orcid":false,"given":"Sara","family":"Bernardoni","sequence":"first","affiliation":[{"name":"Institute of Science Technology and Sustainability for Ceramics (ISSMC) National Research Council (CNR)  Via Granarolo 64 Faenza 48018 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7083-2211","authenticated-orcid":false,"given":"Lucia","family":"Ferrazzano","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cGiacomo Ciamician\u201d Alma Mater Studiorum \u2013 University of Bologna  Via Selmi 2 Bologna 40126 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Palladino","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cGiacomo Ciamician\u201d Alma Mater Studiorum \u2013 University of Bologna  Via Selmi 2 Bologna 40126 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8365-0591","authenticated-orcid":false,"given":"Chiara","family":"Artusi","sequence":"additional","affiliation":[{"name":"Institute of Science Technology and Sustainability for Ceramics (ISSMC) National Research Council (CNR)  Via Granarolo 64 Faenza 48018 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8101-9835","authenticated-orcid":false,"given":"Francesca","family":"Bonvicini","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Biotechnology Alma Mater Studiorum \u2013 University of Bologna  Via Massarenti 9 Bologna 40138 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8931-2921","authenticated-orcid":false,"given":"Elisabetta","family":"Campodoni","sequence":"additional","affiliation":[{"name":"Institute of Science Technology and Sustainability for Ceramics (ISSMC) National Research Council (CNR)  Via Granarolo 64 Faenza 48018 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1608-9364","authenticated-orcid":false,"given":"Giovanna Angela","family":"Gentilomi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Biotechnology Alma Mater Studiorum \u2013 University of Bologna  Via Massarenti 9 Bologna 40138 Italy"},{"name":"Microbiology Unit IRCCS Azienda Ospedaliero\u2010Universitaria di Bologna  Via Massarenti 9 Bologna 40138 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Tolomelli","sequence":"additional","affiliation":[{"name":"Department of Chemistry \u201cGiacomo Ciamician\u201d Alma Mater Studiorum \u2013 University of Bologna  Via Selmi 2 Bologna 40126 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5782-3137","authenticated-orcid":false,"given":"Monica","family":"Sandri","sequence":"additional","affiliation":[{"name":"Institute of Science Technology and Sustainability for Ceramics (ISSMC) National Research Council (CNR)  Via Granarolo 64 Faenza 48018 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,14]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2021.117767"},{"key":"e_1_2_9_2_1","first-page":"258","author":"Torkaman S.","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.11.168"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19092699"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jval.2017.07.007"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2021.0026"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.7603\/s40681-015-0022-9"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2020.115839"},{"key":"e_1_2_9_9_1","first-page":"222","volume":"25","author":"Mndlovu H.","year":"2020","journal-title":"Macromol. Chem."},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/agt2.21"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2018.00137"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.841583"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eurpolymj.2021.110784"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1BM00411E"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/09205063.2022.2088528"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.35086"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajps.2022.01.001"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.00182"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.1260"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.02.140"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.13786"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2020.09.039"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2021.05.013"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03513-14"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm200450h"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm701600a"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41429-021-00406-5"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2015.03.089"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.07.011"},{"key":"e_1_2_9_30_1","first-page":"62","volume":"103","author":"Thapa R. K.","year":"2020","journal-title":"Acta Biomater."},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12929"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.202100103"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24021004"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00080.2022"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.3c00191"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10989-021-10268-y"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14091944"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/milmed\/usz222"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmats.2021.650223"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2018.06.114"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0939-6411(03)00160-7"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.650598"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/app.50781"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2011.01.005"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2015.08.004"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.05.158"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym11122094"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molliq.2014.09.028"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b10491"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2SM00058J"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.350"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2018.10.026"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1520\/JTE11977J"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.0c05604"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2015.1023291"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2015.06.033"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/pat.1625"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.07.034"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2014.09.007"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.03.114"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2021.08.006"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1080\/1539445X.2021.1933032"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/pen.25574"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2021.08.068"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymdegradstab.2015.07.022"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-102192-7.00016-3"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2010.04.048"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2012.04.031"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1021\/ma8021234"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2020.1232"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep24596"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2017.08.052"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.08.169"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00194-12"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2TB00348A"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(01)00248-6"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-019-0514-y"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.9b00072"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.67.4.593-656.2003"}],"container-title":["Macromolecular Bioscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mabi.202400375","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T07:17:46Z","timestamp":1744183066000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mabi.202400375"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,14]]},"references-count":79,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1002\/mabi.202400375"],"URL":"https:\/\/doi.org\/10.1002\/mabi.202400375","archive":["Portico"],"relation":{},"ISSN":["1616-5187","1616-5195"],"issn-type":[{"value":"1616-5187","type":"print"},{"value":"1616-5195","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,14]]},"assertion":[{"value":"2024-08-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2400375"}}