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Recent research highlights biologic\u2010human placental tissue (HPT) as a potential OA therapy due to its biocompatibility, abundant protein biofactors, and ability to reduce cartilage degradation by suppressing protease expression. Microneedles (MNs) are receiving growing attention for enhancing transdermal delivery of biologics as an alternative to conventional subcutaneous injections. The lyophilized human placental extract (LHP) loaded polymeric MNs are fabricated using a micromolding technique for transdermal delivery. Ex vivo release studies reveal that MNs exhibit a gradual and consistent release of LHP, indicating a sustained delivery profile. LHP\u2010MNs are nontoxic and anti\u2010inflammatory in nature against human skin cells and interleukin (IL\u20101\u03b2) induced synovial cells. Furthermore, the in vivo study shows that LHP\u2010MNs substantially improve behavioral parameters in OA rat models and lower serum concentrations of tumor necrosis factor\u2010 \u03b1 (TNF\u2010\u03b1) and cartilage oligomeric matrix protein (COMP) biomarkers, thereby alleviating knee and ankle joint injuries. Histopathological analysis indicates that LHP\u2010MNs significantly preserve cartilage integrity. The study results suggest that employing polymeric MNs for transdermal delivery of LHP can be a promising treatment approach for OA, with the added benefit of excellent patient compliance.<\/jats:p>","DOI":"10.1002\/mabi.202400485","type":"journal-article","created":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T09:29:11Z","timestamp":1734686951000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Polymeric Microneedles for Transdermal Delivery of Human Placental Tissue for the Treatment of Osteoarthritis"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8424-8748","authenticated-orcid":false,"given":"Jithu Jerin","family":"James","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy M S Ramaiah University of Applied Sciences  Bengaluru Karnataka 560054 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8135-0402","authenticated-orcid":false,"given":"Suman","family":"Pahal","sequence":"additional","affiliation":[{"name":"Institute for Stem Cell Science and Regenerative Medicine  Bengaluru Karnataka 560065 India"},{"name":"Institute of Materials Science Polymer Program University of Connecticut  Storrs CT 06269 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7721-0311","authenticated-orcid":false,"given":"Anbu","family":"Jayaraman","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy M S Ramaiah University of Applied Sciences  Bengaluru Karnataka 560054 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3904-4700","authenticated-orcid":false,"given":"Damodar","family":"Nayak A","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy M S Ramaiah University of Applied Sciences  Bengaluru Karnataka 560054 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9701-1615","authenticated-orcid":false,"given":"Sridhar","family":"Koteshwar Narasimhachar","sequence":"additional","affiliation":[{"name":"Cancyte Technologies Pvt Ltd. 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