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This can compromise device functionality and lead to life\u2010threatening complications. High doses of anticoagulants, combined with anti\u2010thrombogenic surface coatings, are typically employed to mitigate this risk, but such treatment can lead to hemorrhagic complications. Therefore, bioactive surface coatings that mimic endothelial blood regulation are needed. However, evaluating these coatings under realistic ECMO conditions is both expensive and challenging. This study utilizes microchannel devices to simulate ECMO fluid dynamics and assess the clot\u2010lysis efficacy of a self\u2010activating fibrinolytic coating system. The system uses antifouling polymer brushes combined with tissue plasminogen activator (tPA) to induce fibrinolysis at the surface. Here, tPA catalyzes the conversion of blood plasminogen into plasmin, which dissolves clots. This positive feedback loop enhances clot digestion under ECMO\u2010like conditions. This findings demonstrate that this coating system can significantly improve the hemocompatibility of medical device surfaces.<\/jats:p>","DOI":"10.1002\/mabi.202400530","type":"journal-article","created":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T11:39:01Z","timestamp":1736768341000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Enhancing Hemocompatibility in ECMO Systems With a Fibrinolytic Interactive Coating: in Vitro Evaluation of Blood Clot Lysis Using a 3D Microfluidic Model"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8388-6756","authenticated-orcid":false,"given":"Lena","family":"Witzdam","sequence":"first","affiliation":[{"name":"Institute for Bioengineering of Catalonia (IBEC) The Barcelona Institute of Science and Technology (BIST)  Carrer de Baldiri Reixac, 10, 12 Barcelona 08028 Spain"},{"name":"DWI \u2013 Leibniz Institute for Interactive Materials  Forckenbeckstr. 50 52074 Aachen Germany"},{"name":"Institute of Technical and Macromolecular Chemistry RWTH Aachen University  Worringerweg 2 52074 Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samarth","family":"Sandhu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Carnegie Mellon University  5000 Forbes Ave Pittsburgh PA 15213 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8323-9919","authenticated-orcid":false,"given":"Suji","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Carnegie Mellon University  5000 Forbes Ave Pittsburgh PA 15213 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9963-6970","authenticated-orcid":false,"given":"Yeahwa","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Carnegie Mellon University  5000 Forbes Ave Pittsburgh PA 15213 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanzeh","family":"Kamal","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Carnegie Mellon University  5000 Forbes Ave Pittsburgh PA 15213 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0722-3122","authenticated-orcid":false,"given":"Oliver","family":"Grottke","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology RWTH Aachen University Hospital  Pauwelsstr. 30 52074 Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5604-3718","authenticated-orcid":false,"given":"Keith E.","family":"Cook","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering Carnegie Mellon University  5000 Forbes Ave Pittsburgh PA 15213 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0745-0840","authenticated-orcid":false,"given":"Cesar","family":"Rodriguez\u2010Emmenegger","sequence":"additional","affiliation":[{"name":"Institute for Bioengineering of Catalonia (IBEC) The Barcelona Institute of Science and Technology (BIST)  Carrer de Baldiri Reixac, 10, 12 Barcelona 08028 Spain"},{"name":"DWI \u2013 Leibniz Institute for Interactive Materials  Forckenbeckstr. 50 52074 Aachen Germany"},{"name":"Instituci\u00f3 Catalana de Recerca i Estudis Avan\u00e7ats (ICREA)  Passeig Llu\u00eds Companys 23 Barcelona 08010 Spain"},{"name":"Biomedical Research Networking Center in Bioengineering Biomaterials and Nanomedicine The Institute of Health Carlos III  Av. 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