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In the present study, a PVA\u20104\u2010azidobenzoic acid (AZ)\u2010RGD scaffold is prepared based on cross\u2010linking of two distinct PVA derivatives: one featuring photoreactive azides for ultraviolet (UV)\u2010crosslinking and the other incorporating RGD peptides. The results show that the PVA\u2010AZ\u2010RGD scaffold has good blood compatibility and biomechanical properties, with hydrophilic properties, and a hydrolysis rate of 27.31% at 12 weeks. Notably, the incorporation of RGD peptides significantly bolsters the attachment and proliferation of mesenchymal stem cells (MSCs) on the scaffolds, compared to non\u2010RGD\u2010conjugated controls. Furthermore, RGD conjugation markedly accelerates endothelialization of MSCs following 15 days of endothelial culture. Post\u2010transplantation, the PVA\u2010AZ\u2010RGD scaffold exhibits favorable blood flow patency, minimal immune rejection, promotes endothelialization and smooth muscle cell proliferation, and facilitates the development of extracellular matrix, ultimately contributing to the formation of regenerative artificial blood vessels. These comprehensive findings underscore the promising potential of RGD\u2010integrated, crosslinked PVA scaffolds for applications in vascular tissue engineering.<\/jats:p>","DOI":"10.1002\/mabi.202400554","type":"journal-article","created":{"date-parts":[[2025,2,22]],"date-time":"2025-02-22T13:40:31Z","timestamp":1740231631000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preparation and Evaluation of RGD\u2010Conjugated Crosslinked PVA Tissue Engineered Vascular Scaffold with Endothelial Differentiation and Its Impact on Vascular Regeneration In Vivo"],"prefix":"10.1002","volume":"25","author":[{"given":"Xiafei","family":"Li","sequence":"first","affiliation":[{"name":"College of Medical Engineering Xinxiang Medical University  Xinxiang 453003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuewei","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science 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