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Recent advancements have led to the creation of therapeutic dressings incorporating drug\u2010delivery systems to tackle these issues. Exosomes (Exos) derived from mesenchymal stem cells (MSCs) have gained significant attention for mediating therapy without directly using cells, thanks to their natural anti\u2010inflammatory and tissue repair properties mirroring those of MSCs. In this study, an advanced wound dressing combines chitosan (CS) and polyethylene glycol (PEG) hydrogel with adipose MSCs\u2010derived Exos (ADMSCs\u2010Exos). This composite, formed using a straightforward blending technique, is engineered to improve the healing process of severe skin injuries by steadily releasing Exos as the hydrogel degrades. The in vitro studies demonstrate that this hydrogel\u2010exosome dressing greatly enhances endothelial cell migration, reduces oxidative stress, and promotes angiogenesis, crucial for effective wound healing. Additionally, real time\u2010polymerase chain reaction (RT\u2010PCR) analysis revealed significant upregulation of key genes involved in these processes, supporting the therapeutic potential of the hydrogel\u2010Exo combination. These findings emphasize the potential of this hydrogel\u2010Exos combination as an innovative and promising solution for advanced wound care.<\/jats:p>","DOI":"10.1002\/mabi.202400609","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T06:56:02Z","timestamp":1737528962000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["In Vitro Assessment of Chitosan\u2010PEG Hydrogels Enriched with MSCs\u2010Exosomes for Enhancing Wound Healing"],"prefix":"10.1002","volume":"25","author":[{"given":"Masoumeh","family":"Ezati","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering Faculty of Electrical Engineering and Communication Brno University of Technology  Technicka 3082\/12 Brno 61600 Czech 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