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However, the tedious polymer synthesis and the stability issue greatly limit their further development. Herein the biphasic polymerization platform is reported for the easy access of a series of crosslinked PEG\u2010polypeptide nanoparticles for enhanced drug loading and selective drug release. The accelerated polymerization allows for the preparation of a library of PEG\u2010polypeptides from crude monomers with different polypeptide segments for structural optimization. Nanocarriers with redox\u2010responsive de\u2010crosslinking and enhanced drug loading are therefore obtained based on the model drug, doxorubicin, which exhibits selective killing of cancer cells. This study provides a synthetic method for efficient preparation of PEG\u2010polypeptide nanocarriers, benefiting the downstream drug loading and therapeutic studies.<\/jats:p>","DOI":"10.1002\/mabi.202500063","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T18:44:50Z","timestamp":1744397090000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Facile Preparation of Crosslinked PEG\u2010Polypeptide Drug Nanocarriers Through Biphasic Polymerization of Crude <i>N<\/i>\u2010Carboxyanhydrides"],"prefix":"10.1002","author":[{"given":"Shijie","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Functional Nano &amp; Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon\u2010Based Functional Materials and Devices Soochow University Suzhou 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanshan","family":"Xiao","sequence":"additional","affiliation":[{"name":"Institute of Functional Nano &amp; Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon\u2010Based Functional Materials and Devices Soochow University Suzhou 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Functional Nano &amp; Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon\u2010Based Functional Materials and Devices Soochow University Suzhou 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Functional Nano &amp; Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon\u2010Based Functional Materials and Devices Soochow University Suzhou 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3165-3712","authenticated-orcid":false,"given":"Ziyuan","family":"Song","sequence":"additional","affiliation":[{"name":"Institute of Functional Nano &amp; Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon\u2010Based Functional Materials and Devices Soochow University Suzhou 215123 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1038\/nrd2591","volume":"9","author":"Petros R. 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