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However, other ingredients can also influence hydrogel formation and performance. Therefore, this study examines the effect of different salt molarities in phosphate buffered saline (PBS) solutions, used as reaction solvents, on the properties of hydrogels mainly composed of polyethylene glycol, agarose, and carbomer. To conduct the study, two PBS solutions are prepared by varying the salt concentrations, and these mixtures are used as solvents in a bulk polycondensation reaction. Subsequently, the two hydrogel formulations obtained are characterized to analyze the effect of PBS concentration on the material properties. An increase in salt concentration reduced the swelling capacity and release ability of the hydrogel matrix, as well as decreased the porosity and interconnectivity of the material, contributing to non\u2010homogeneity in the pore network. Consequently, these results highlight that the salinity and ionic strength of buffering solutions can affect various material properties, and therefore, careful consideration should be given during the preparation of these solutions.<\/jats:p>","DOI":"10.1002\/mabi.202500073","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T11:56:39Z","timestamp":1743767799000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Exploring the Role of Aqueous Buffered Saline Solutions on the Macroscopic Properties of PEG\/Carbomer\/Agarose Hydrogels"],"prefix":"10.1002","volume":"25","author":[{"given":"Zoe","family":"Giorgi","sequence":"first","affiliation":[{"name":"Department of Chemistry Materials and Chemical Engineering \u201cGiulio Natta\u201d Politecnico di Milano  via Mancinelli 7 Milano 20131 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