{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T10:03:37Z","timestamp":1775469817716,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,8,17]],"date-time":"2025-08-17T00:00:00Z","timestamp":1755388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>T cells in the lymph nodes play an important role in cancer immunotherapy. Dendrigraft polylysines (DGLs) are potent nanoplatforms used in nanomedicine. In the present study, DGLs were modified with 1,2\u2010cyclohexanedicarboxylic acid (CHex) and phenylalanine (Phe) to produce DGL\u2010CHex\u2010Phe for drug delivery into T cells. Various DGL\u2010CHex\u2010Phe polymers were synthesized using different generations of DGL by reacting with Phe at different ratios. DGL\u2010CHex\u2010Phe polymers with a higher generation and more Phe efficiently associated with Jurkat cells, a T cell model. These polymers are internalized by T cells via an amino acid transporter and\/or direct membrane association. The hydrophobic model drug, paclitaxel (PTX), was loaded onto the polymers. DGL(G3)\u2010CHex\u2010Phe93 loaded the most PTX molecules among them, and most of them were retained therein for 3\u00a0h. PTX\u2010loaded polymers exhibited cytotoxic effects against Jurkat cells at a level similar to that of free PTX. DGL(G3)\u2010CHex\u2010Phe93 efficiently accumulated in lymph nodes after intradermal injection, which was partially co\u2010localized with T cells. These results suggested that DGL(G3)\u2010CHex\u2010Phe93 is useful for the delivery of hydrophobic drugs into immune cells including T cells in lymph nodes.<\/jats:p>","DOI":"10.1002\/mabi.202500207","type":"journal-article","created":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T03:24:19Z","timestamp":1755487459000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Hydrophobic Drug Delivery into T Cells Using Carboxy\u2010Terminal Phenylalanine\u2010Modified Dendrigraft Polylysines"],"prefix":"10.1002","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2208-5784","authenticated-orcid":false,"given":"Chie","family":"Kojima","sequence":"first","affiliation":[{"name":"Department of Materials Science and Engineering School of Materials and Chemical Technology Institute of Science Tokyo  Yokohama Kanagawa Japan"},{"name":"Department of Applied Chemistry Graduate School of Engineering Osaka Metropolitan University  Sakai Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akinobu","family":"Sakai","sequence":"additional","affiliation":[{"name":"Department of Materials Science and Engineering School of Materials and Chemical Technology Institute of Science Tokyo  Yokohama Kanagawa Japan"},{"name":"Department of Applied Chemistry Graduate School of Engineering Osaka Metropolitan University  Sakai Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Kadonosono","sequence":"additional","affiliation":[{"name":"Department of Life Science and Technology School of Life Science and Technology Institute of Science Tokyo  Yokohama Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-0306-5"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2022.101621"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10673"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.07.012"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-020-00822-y"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3186"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7CS00550D"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2018.07.030"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2018.07.020"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2005.09.018"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym12071474"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2015.08.002"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1TB01980E"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics15030888"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics16060715"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.202300139"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2021.113456"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201704147"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/09205063.2015.1095023"},{"key":"e_1_2_8_21_1","first-page":"20143","article-title":"Dendritic Polylysine with Paclitaxel and Triptolide Codelivery for Enhanced Cancer Ferroptosis through the Accumulation of ROS","volume":"16","author":"Wen Y.","year":"2024","journal-title":"ACS Applied Materials & Interfaces"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2020.110895"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsapm.1c01354"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14112348"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2024.102340"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-020-02826-8"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04366-7"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/ma302125f"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp508473w"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.7b09219"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2020.10.004"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2020.119021"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2021.112159"}],"container-title":["Macromolecular Bioscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mabi.202500207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T16:43:30Z","timestamp":1763138610000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mabi.202500207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,17]]},"references-count":32,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/mabi.202500207"],"URL":"https:\/\/doi.org\/10.1002\/mabi.202500207","archive":["Portico"],"relation":{},"ISSN":["1616-5187","1616-5195"],"issn-type":[{"value":"1616-5187","type":"print"},{"value":"1616-5195","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,17]]},"assertion":[{"value":"2025-03-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00207"}}