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However, existing surgical options are invasive and lack ideal biomaterials for scaffold\u2010based healing. Herein, we present a custom\u2010engineered mechano\u2010acoustic responsive hydrogel incorporating bFGF\u2010loaded sodium alginate microspheres, designed for controlled drug release and tissue regeneration under dual stimulation: vibrational simulation and low\u2010frequency acoustic waves (generated by radio devices) \u2014 representing its first transition from in vitro characterization to in vivo regenerative application. This polymer\u2010based scaffold exhibits robust adhesion, biocompatibility, and mechanoresponsive release behavior. A rat acute TMP model was employed to evaluate the hydrogel's therapeutic efficacy. Compared with control and blank hydrogel groups, the bFGF\u2010loaded mechanoresponsive hydrogel (SGM) noticeably accelerated tympanic membrane closure, reduced local inflammation, and enhanced early auditory recovery, as confirmed by otoscopic inspection, ABR tests, histological staining, and TEM imaging. Our findings demonstrate that the SGM hydrogel effectively promotes functional tissue regeneration and early hearing restoration in vivo. This work highlights the potential of polymer\u2010based, stimuli\u2010responsive biomaterials in advancing minimally invasive strategies for TMP treatment and offers valuable insights for future tissue engineering applications in otolaryngology.<\/jats:p>","DOI":"10.1002\/mabi.202500261","type":"journal-article","created":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T03:14:27Z","timestamp":1759288467000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of a Photopolymerized Gelatin Hydrogel Network with bFGF\u2010Loaded Alginate Microspheres for Tympanic Membrane Perforation Repair in Rats"],"prefix":"10.1002","volume":"25","author":[{"given":"Hao","family":"Xue","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery The First Affiliated Hospital of Ningbo University Ningbo Zhejiang P. R. 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