{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:33:34Z","timestamp":1790026414828,"version":"4.0.1"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002560","name":"Soonchunhyang University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002560","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Polydeoxyribonucleotide (PDRN), a bioactive DNA fragment, has been known to promote anti\u2010inflammatory responses and wound healing primarily via adenosine A\n                    <jats:sub>2<\/jats:sub>\n                    A receptor activation. However, low molecular weight PDRN can undergo rapid degradation, limiting its sustained therapeutic efficacy. In this study, we developed a scalable method to produce high\u2010purity, and low molecular weight PDRN (\n                    <jats:italic>c.a<\/jats:italic>\n                    . 325\u00a0bp) from calf thymus DNA via physical fragmentation. To enhance its stability and delivery, PDRN was encapsulated in poly(lactic\u2010co\u2010glycolic acid) (PLGA) to form PDRN\/PLGA nanoparticles, yielding uniform and spherical particles (336\u00a0\u00b1\u00a043\u00a0nm). These nanoparticles exhibited excellent colloidal stability and biodegradability (38.3% over 14 days), with sustained PDRN release (88.39% over 14 days). Moreover, the nanoformulation effectively protected PDRN from thermal, acidic, enzymatic, and UV degradation. The PDRN\/PLGA nanoparticles, which exhibited no cytotoxicity or hemolysis, demonstrated superior anti\u2010inflammatory and wound\u2010healing efficacy compared to free PDRN. In an\n                    <jats:italic>in vitro<\/jats:italic>\n                    lipopolysaccharide (LPS)\u2010induced inflammatory wound model, they significantly accelerated wound closure compared to both LPS\u2010treated and untreated controls. These results suggest that nanoformulation effectively protects low molecular weight PDRN, thereby significantly enhancing its therapeutic activity and underscore the potential of PDRN\/PLGA nanoparticles as a stable and effective platform for the regeneration of skin inflammation.\n                  <\/jats:p>","DOI":"10.1002\/mabi.202500373","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T08:36:47Z","timestamp":1768379807000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Nanoformulated PDRN Improves Anti\u2010Inflammatory and Wound Healing Activities"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0404-1626","authenticated-orcid":false,"given":"Ji\u2010Hye","family":"Kang","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology College of Medical Science Soonchunhyang University Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4233-9623","authenticated-orcid":false,"given":"Min Jeong","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Medical Science College of Medical Science Soonchunhyang University Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1469-1100","authenticated-orcid":false,"given":"Sung\u2010Eun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Medical Science College of Medical Science Soonchunhyang University Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won Kyung","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Medical Science College of Medical Science Soonchunhyang University Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi\u2010Young","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology College of Medical Science Soonchunhyang University Asan Chungnam Republic of Korea"},{"name":"Department of Medical Science College of Medical Science Soonchunhyang University Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/08830180600785868"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2007.02718.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-02803-4"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10112853"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21737"},{"issue":"3","key":"e_1_2_9_7_1","first-page":"224","article-title":"Nucleic Acid Aptamers for Target Validation and Therapeutic Applications","volume":"16","author":"Pendergrast P. 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