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While gelatin is highly water\u2010soluble, it does not form the triple\u2010helical structure. As a result, physically crosslinked gelatin\u2010based hydrogels are only stable at low temperatures, precluding their use in 3D cell culture. Gelatin\u2010methacryloyl (GelMA) and gelatin\u2010norbornene (GelNB) have been developed to enable the stable crosslinking of gelatin\u2010based hydrogels via chain\u2010growth or step\u2010growth photopolymerization. However, most gelatin\u2010based hydrogels lack dynamically tunable properties unless macromers with dynamically crosslinkable motifs are used. Here, we integrate GelNB with dithiolane\u2010containing crosslinker poly(ethylene glycol)\u2010tetra\u2010lipoic acid (PEG4LA)\u2010for modular photo\u2010crosslinking of GelNB into hydrogels under cytocompatible light exposure (365 nm, 5 mW\/cm\n                    <jats:sup>2<\/jats:sup>\n                    ) with a low photoinitiator concentration (1 m\n                    <jats:sc>m<\/jats:sc>\n                    LAP). Even under these mild reaction conditions, the stiffness of GelNB\/PEG4LA hydrogels could be dynamically tuned by inducing dithiolane ring\u2010opening via secondary light exposure, thereby creating dynamic and cytocompatible hydrogels suitable for in situ encapsulation, culture, and differentiation of human induced pluripotent stem cells (hiPSCs).\n                  <\/jats:p>","DOI":"10.1002\/mabi.202500400","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T04:22:30Z","timestamp":1770178950000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Dynamic Gelatin Hydrogels Crosslinked by Dithiolane\u2010Norbornene Click Chemistry"],"prefix":"10.1002","volume":"26","author":[{"given":"Favour O.","family":"Afolabi","sequence":"first","affiliation":[{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia Yang","family":"He","sequence":"additional","affiliation":[{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4175-8796","authenticated-orcid":false,"given":"Chien\u2010Chi","family":"Lin","sequence":"additional","affiliation":[{"name":"Weldon School of Biomedical Engineering Purdue University  West Lafayette Indiana 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