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Mistreatment of these wound infections often increases antimicrobial resistance (AMR), thereby decreasing the effectiveness of antibiotics. With the increase in AMR, new antimicrobial therapeutics that target these hard\u2010to\u2010kill pathogens are needed. Herein, naturally harvested bacteriophages (EC\u03a6) were combined with another established antimicrobial molecule, nitric oxide (NO). This combination has rarely been explored in biomedical devices but shows excellent potential for developing broad\u2010spectrum, antibacterial materials. Bacteriophages were encapsulated in alginate microbeads and suspended in a NO\u2010releasing thermoresponsive hydrogel. The phages were shown to have a delayed release from the alginate beads when incorporated into the gel, compared to the release observed within 24\u00a0h in aqueous medium. This delayed release enabled tunable phage delivery by adjusting the viscosity of the bulk gel base. Additionally, we used alginate as the base for microbeads, resulting in a physiologically safe material due to its proven biocompatibility. The final EC\u03a6 and NO\u2010releasing bead\u2010gel matrix demonstrated 5\u201310 times larger zones of bacterial killing while maintaining low cytotoxicity, enabling further development in various clinical applications, including wound healing.<\/jats:p>","DOI":"10.1002\/mabi.202500568","type":"journal-article","created":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T11:52:11Z","timestamp":1769860331000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bacteriophage and Nitric Oxide Combined Release from a Dual Hydrogel Matrix for Wound Healing Applications"],"prefix":"10.1002","volume":"26","author":[{"given":"Sarah N.","family":"Wilson","sequence":"first","affiliation":[{"name":"School of Chemical Materials, and Biological Engineering College of Engineering University of Georgia  Athens Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam 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