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Pre\u2010transplant conditioning strategies that support physiological homeostasis while protecting islets within encapsulation devices are therefore essential. We developed electrospun hydrophilic cellulose acetate (eCA) membranes and hydrophobic polytetrafluoroethylene (ePTFE) membranes with nano\u2010topographical surfaces for islet encapsulation. They were tested for protein adsorption and macrophage activation, while eCA and ePTFE devices were evaluated by encapsulating MIN6 spheroids to assess their encapsulation efficiency and Glucose\u2010stimulated insulin secretion (GSIS) capability. Furthermore, the devices encapsulating MIN6 spheroids were tested for 14 days in a custom 3D\u2010printed bioreactor with continuous dynamic flow. Both eCA and ePTFE membranes restricted the entry or attachment of activated macrophages. The cells encapsulated in eCA\u2010devices released significantly more insulin than those in ePTFE\u2010devices, reflected by higher stimulation indices, suggesting the nutrient transfer ability of eCA. Under dynamic conditions, the encapsulated spheroids in eCA\u2010devices were associated with high viability (80% at day 7 and 98% at day 14) and underwent initial compaction followed by tissue\u2010like structure formation. Microscopy and immunofluorescence revealed the presence of ECM proteins, collagen\u20101, and E\u2010cadherin, supporting the compaction and remodeling. These results demonstrate that the bioreactor system may be utilised as a pre\u2010transplantation conditioning platform to rehabilitate isolated islets within encapsulated devices.<\/jats:p>","DOI":"10.1002\/mabi.202500603","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T14:21:54Z","timestamp":1768918914000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Maturation of Pancreatic Spheroids within Macroencapsulation Devices to Tissue\u2010Like Structures by a 3D\u2010Printed Bioreactor"],"prefix":"10.1002","volume":"26","author":[{"given":"Gokula Nathan","family":"Kasinathan","sequence":"first","affiliation":[{"name":"Regenerative Medicine and Stem Cell Laboratory (RMS) Department of Biomedical Engineering Indian Institute of Technology Hyderabad  Sangareddy Telangana 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