{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:15:57Z","timestamp":1790885757442,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Advanced biomaterials\u2010based\n                    <jats:italic>in vitro<\/jats:italic>\n                    platforms are increasingly required to overcome the limited predictive power of conventional 2D cell cultures in colorectal cancer (CRC) drug screening. Herein, we report the development of a biomimetic, multicellular 3D CRC model based on gelatin methacrylate (GelMA) hydrogels, designed to recapitulate key structural and biological features of the tumor microenvironment. The platform integrates a vascularized hydrogel compartment with human endothelial cells, combined with cancer\u2010associated fibroblasts and macrophages, enabling controlled tumor\u2010stroma\u2010vessel interactions within a physiologically relevant architecture. The GelMA hydrogels were comprehensively characterized, and their role in regulating endothelial viability, migration, and angiogenic marker expression was systematically evaluated. The drug screening capability of the platform was assessed using 5\u2010fluorouracil (5\u2010FU). Comparative analyses revealed that 3D cultures exhibited attenuated cytotoxicity, oxidative stress, and apoptotic responses relative to 2D monolayers, particularly at lower drug concentrations and prolonged exposure times. These findings demonstrate that GelMA\u2010based microenvironment actively modulates cellular drug responses through multicellular interactions and diffusion\u2010mediated effects, rather than acting as a passive scaffold. Overall, this study establishes a functional 3D\n                    <jats:italic>in vitro<\/jats:italic>\n                    platform that provides improved physiological relevance and predictive capability for preclinical CRC drug screening, while offering a human\u2010relevant alternative aligned with the principles of the 3Rs.\n                  <\/jats:p>","DOI":"10.1002\/mabi.70190","type":"journal-article","created":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T18:05:02Z","timestamp":1777658702000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Multicellular 3D GelMA\u2010Based Colorectal Cancer Model for Chemotherapeutic Responses"],"prefix":"10.1002","volume":"26","author":[{"given":"Seyfure","family":"Ad\u0131g\u00fczel","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics University of Health Sciences Turkey  Istanbul T\u00fcrkiye"},{"name":"Experimental 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T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8757-0572","authenticated-orcid":false,"given":"Merve G\u00fcd\u00fcl","family":"Bacanl\u0131","sequence":"additional","affiliation":[{"name":"G\u00fclhane Faculty of Pharmacy Department of Pharmaceutical Toxicology University of Health Sciences Turkey  Ankara T\u00fcrkiye"},{"name":"Turkish Vaccine Institute Health Institutes of T\u00fcrkiye (T\u00dcSEB) Aziz Sancar Research Center  Ankara 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