{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:17:13Z","timestamp":1787840233108,"version":"build-2784847793"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004809","name":"Financiadora de Estudos e Projetos","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Macromolecular Bioscience"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Degenerative ocular diseases are among the leading causes of visual impairment worldwide and require innovative therapeutic approaches capable of promoting tissue regeneration. In this context, biomaterial\u2010based cell therapies have emerged as promising alternatives, although challenges remain regarding the maintenance of a supportive microenvironment after transplantation. This study developed and characterized a hybrid biomembrane composed of poly(lactic\n                    <jats:italic>\u2010co\u2010<\/jats:italic>\n                    glycolic acid) (PLGA) and decellularized corneal extracellular matrix (dCECM) to provide structural and biochemical support for ocular progenitor cells. PLGA membranes were fabricated and coated with dCECM layers. Their morphological, physicochemical, mechanical, and biocompatibility properties were evaluated. Biocompatibility was assessed using stem cells from the apical papilla (SCAP), periodontal ligament (PDLC), and corneal stroma (CSSC). The capacity of the membranes to preserve ocular progenitor markers (PAX6 and RX) and to exert cytoprotective effects on oxidatively damaged retinal pigment epithelial (RPE) cells was also investigated. Hybrid PLGA\u2010dCECM membranes exhibited suitable thickness, increased hydrophilicity, improved tensile strength, and enhanced cell adhesion without evidence of chemical incompatibility. SCAP and CSSC maintained high expression of ocular progenitor markers, while co\u2010culture with injured RPE cells reduced LDH release and cell death. The PLGA\u2010dCECM biomembrane showed both structural support and bioactive cues, supporting its potential application in ocular regenerative therapies.\n                  <\/jats:p>","DOI":"10.1002\/mabi.70231","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T04:37:16Z","timestamp":1785991036000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and Characterization of a Structurally Supportive of Hybrid PLGA Decellularized ECM Biomembrane for Ocular Progenitor Cell Anchorage and Epithelial Repair"],"prefix":"10.1002","volume":"26","author":[{"given":"Ana Karulline Garcia","family":"Ungaratti","sequence":"first","affiliation":[{"name":"Laboratory of Education and Research in In Vitro Toxicology (Tox In) Faculty of Pharmacy Federal University of Goi\u00e1s  Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aliny Pereira","family":"de Lima","sequence":"additional","affiliation":[{"name":"Laboratory of Education and Research in In Vitro Toxicology (Tox In) Faculty of Pharmacy Federal University of Goi\u00e1s  Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordana Andrade","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Education and Research in In Vitro Toxicology (Tox In) Faculty of Pharmacy Federal University of Goi\u00e1s  Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Leticia","family":"de Melo","sequence":"additional","affiliation":[{"name":"Laboratory of Education and Research in In Vitro Toxicology (Tox In) Faculty of Pharmacy Federal University of Goi\u00e1s  Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Graziani","sequence":"additional","affiliation":[{"name":"Multiuser Laboratory for the Evaluation of Molecules, Cells, and Tissues School of Veterinary Medicine and Animal Science Federal University of Goi\u00e1s  Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7790-9771","authenticated-orcid":false,"given":"Artur Christian G.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Education and Research in In Vitro Toxicology (Tox In) Faculty of Pharmacy Federal University of Goi\u00e1s  Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Ant\u00f4nio Dantas","family":"Silva","sequence":"additional","affiliation":[{"name":"Center For Research Technological Development and Innovation in Drugs Medicines and Cosmetics Federal University of Goi\u00e1s  Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0379-1325","authenticated-orcid":false,"given":"Marize Campos","family":"Valadares","sequence":"additional","affiliation":[{"name":"Laboratory of Education and Research in In Vitro Toxicology (Tox In) Faculty of Pharmacy Federal University of Goi\u00e1s  Goi\u00e2nia Goi\u00e1s Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-020-10090-x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aopr.2024.02.001"},{"key":"e_1_2_8_4_1","article-title":"Molecular and Cellular Mechanisms of the Therapeutic Effect of Mesenchymal Stem Cells and Extracellular Vesicles in Corneal Regeneration","volume":"25","author":"Marzidov\u0161ek Z. 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