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Surface modification of polymeric materials is an effective strategy for controlling the anti\u2010fouling properties of biomedical materials. Synthetic polymers such as poly(ethylene glycol) (PEG), which exhibit high bioinertness and anti\u2010fouling properties, are widely used. Peptide\u2010based anti\u2010fouling materials are gaining attention because of their biocompatibility, long\u2010term biodegradability, and structural diversity. In this study, we explored the potential of antifreeze protein type I (AFP) from winter flounder as a peptide\u2010based anti\u2010fouling material. AFP possesses a simple \u03b1\u2010helical structure of approximately 30 amino acid residues and presents an amphiphilic interface consisting of an ice\u2010binding surface and a hydrophilic surface. We constructed a recombinant AFP that can be modified on a glass substrate and evaluated its anti\u2010fouling properties. The AFP\u2010modified substrates significantly suppressed the adsorption of plasma proteins, such as human albumin, fibrinogen, and fibronectin. Furthermore, adhesion of human ovarian cancer SKOV3 cells and mouse platelets to the substrates was suppressed by AFP modification. Notably, tandem\u2010repeat AFP constructs exhibited high resistance to proteolytic enzymes, such as trypsin. These findings highlight the potential of recombinant AFP as a potential peptide\u2010based anti\u2010fouling material for biomedical applications.<\/jats:p>","DOI":"10.1002\/mabi.70235","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T04:25:41Z","timestamp":1785990341000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Recombinant Antifreeze Protein Type I as a Potential Peptide\u2010Based Anti\u2010Fouling Material for Biomedical Surfaces"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6493-5436","authenticated-orcid":false,"given":"Kei","family":"Nishida","sequence":"first","affiliation":[{"name":"Department of Life Science and Technology School of Life Science and Technology Institute of Science Tokyo  Yokohama Japan"},{"name":"Graduate School of Advanced Science and Technology Japan Advanced Institute of Science and Technology Ishikawa 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