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In this study, a methacrylated gelatin (GelMA) and methacrylated chitosan (CSMA) composite hydrogel coating is developed on poly(phthalazinone ether nitrile ketone) (PPENK), showing pro\u2010osteogenic and anti\u2010osteoclastic effects in vitro. Nano\u2010zinc oxide (nano\u2010ZnO), nano\u2010hydroxyapatite (nano\u2010HA), and phosphate\u2010ester\u2010functionalized alendronate (ALNP) are incorporated to confer antibacterial activity, promote osteogenesis, and enable enzyme\u2010responsive release of anti\u2010osteoporosis alendronate (ALN). SEM\/EDS results confirm the homogeneous dispersion of the nanoparticles within the porous hydrogel. The stability of the hydrogel coating is verified by smooth scratch edges and no visible detachment in the cross\u2010hatch test. ALN release experiments confirm that the release amount of ALN is positively correlated with alkaline phosphatase (ALP) concentration, indicating significant enzyme\u2010responsive characteristics. In vitro studies show that the hydrogel coating on PPENK exhibits antibacterial activity. Nano\u2010HA and nano\u2010ZnO enhance the expression of osteogenic\u2010related genes and proteins in MC3T3\u2010E1 pre\u2010osteoblasts. The released ALN inhibits the receptor activator of nuclear factor\u2010\u03baB ligand (RANKL)\u2010induced differentiation of RAW264.7 macrophages into osteoclasts. Overall, this surface modification strategy integrates enzyme\u2010responsive ALN release with composite hydrogel coating on PPENK, realizing antibacterial, osteogenic, and anti\u2010osteoclastic effects in vitro.<\/jats:p>","DOI":"10.1002\/mabi.70258","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T18:56:36Z","timestamp":1789498596000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enzyme\u2010Responsive Composite Hydrogel Coating Containing Nano\u2010HA\/Nano\u2010ZnO with Pro\u2010Osteogenic and Anti\u2010Osteoclastic Properties"],"prefix":"10.1002","volume":"26","author":[{"given":"Yue","family":"Yu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Fine Chemicals Dalian University of Technology  Dalian China"},{"name":"Department of Polymer Science &amp; Materials Dalian University of Technology  Dalian 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