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Rapid Commun."],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Along with the quick advancements in enzyme technology, inactivation has emerged as the key barrier for enzymes to be fully utilized as biocatalysts. Here, a novel strategy is presented for the preservation of the enzymatic activity even after heat treatment by grafting enzymes onto the thermal responsive block copolymer via an activated ester\u2010amine reaction. A new water\u2010soluble activated ester monomer, acrylic polyethylene glycol (PEG) functionalized 3\u2010fluoro\u20104\u2010hydroxybenzoate is synthesized. This activated ester monomer and 2\u2010methoxyethoxyethyl methacrylate (MEEMA) as copolymer monomers are first used to synthesize water\u2010soluble polymers bearing activated ester for post\u2010polymerization modification with amines. Two model enzymes containing amine residues, urease, and papain, are grafted onto the resulting thermal responsive polymers to obtain PMEEMA\u2010co\u2010Enzyme, respectively. The obtained particles of polymer\u2010enzyme conjugates flocculate above the low critical solution temperature (LCST)\u00a0and redissolve when cooled below that temperature. The activity of the conjugated enzymes has been studied after high temperatures treatment and compared to that of free enzymes. The enzymatic activity assays show that the thermosensitive polymer can act as a stabilizer under high\u2010temperature conditions after multipoint grafting with the enzyme, thus protecting the enzyme from thermal inactivation.<\/jats:p>","DOI":"10.1002\/marc.202400773","type":"journal-article","created":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T11:19:05Z","timestamp":1736767145000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Protection of Enzymes Against Heat Inactivation by Enzyme\u2013Polymer Conjugates"],"prefix":"10.1002","volume":"46","author":[{"given":"Zhongguo","family":"Jiang","sequence":"first","affiliation":[{"name":"State Key Lab of Polymer Materials Engineering School of Chemical Engineering Sichuan University  Chengdu 610065 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lirong","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Lab of 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