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Following deprotection, the PAE copolymers containing discoverable secondary amino groups demonstrate a preferent bactericidal effect against Gram\u2010negative bacteria. The preference arises from the capacity of PAE copolymers to permeabilize the bacterial outer membrane, subsequently slightly compromising the inner membrane and resulting in the death of Gram\u2010negative bacteria. Furthermore, the PAE copolymers exhibit a comparatively low hemolysis and cytotoxicity.<\/jats:p>","DOI":"10.1002\/marc.202400885","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T17:16:44Z","timestamp":1741713404000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Enzymatic Synthesis of Poly(\u03b2\u2010Amino Ester) Copolymer With High Potency in Eliminating Gram\u2010Negative Bacteria"],"prefix":"10.1002","volume":"46","author":[{"given":"Peng","family":"Dong","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application State Key Laboratory of Applied Microbiology Southern China Institute of Microbiology Guangdong Academy of Sciences  Guangzhou 510070 P. R. 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