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This modification, which has been known for more than a century, occurs to both the free form of the amino acid (i.e., soluble\/free tyrosine) and to tyrosine residues covalently bound within the backbone of peptides and proteins. Nitration of tyrosine is thought to be of biological significance and has been linked to health and disease, but determining its role has proved challenging. Several key questions have been the focus of much of the research activity: (a) to what extent is free\/soluble tyrosine nitrated in biological tissues and fluids, and (b) are there specific site(s) of nitration within peptides\/proteins and to what extent (i.e., stoichiometry) does this modification occur? These issues have been addressed in a wide range of sample types (e.g., blood, urine, CSF, exhaled breath condensate and various tissues) and a diverse array of physiological\/pathophysiological scenarios. The accurate determination of nitrated tyrosine is, however, a stumbling block. Despite extensive study, the extent to which nitration occurs <jats:italic>in vivo<\/jats:italic>, the specificity of the nitration reaction, and its importance in health and disease, remain unclear. In this review, we highlight the analytical challenges and discuss the approaches adopted to address them. Mass spectrometry, in combination with either gas chromatography (GC\u2010MS, GC\u2010MS\/MS) or liquid chromatography (LC\u2010MS\/MS), has played the central role in the analysis of 3\u2010nitrotyrosine and tyrosine\u2010nitrated biological macromolecules. We discuss its unique attributes and highlight the role of stable\u2010isotope labeled 3\u2010nitrotyrosine analogs in both accurate quantification, and in helping to define the biological relevance of tyrosine nitration. We show that the application of sophisticated mass spectrometric techniques is advantageous if not essential, but that this alone is by no means a guarantee of accurate findings. We discuss the important analytical challenges in quantifying 3\u2010nitrotyrosine, possible workarounds, and we attempt to make sense of the disparate findings that have been reported so far. \u00a9 2013 Wiley Periodicals, Inc. 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