{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T15:35:45Z","timestamp":1787931345086,"version":"build-2784847793"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T00:00:00Z","timestamp":1583366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Listeria monocytogene<\/jats:italic>s is the causative agent of human listeriosis which has high hospitalization and mortality rates for individuals with weakened immune systems. The survival and dissemination of <jats:italic>L.\u00a0monocytogenes<\/jats:italic> in adverse environments can be reinforced by the formation of biofilms. Therefore, this study aimed to understand the mechanisms underlying listerial biofilm development. Given that both nutrient availability and quorum sensing (QS) have been known as the factors influencing biofilm development, we hypothesized that the signal from a sentinel metabolite <jats:italic>S<\/jats:italic>\u2010adenosylmethionine (SAM) and Agr\u2010based QS could be synchronous in <jats:italic>L.\u00a0monocytogenes<\/jats:italic> to modulate nutrient availability, the synthesis of extracellular polymeric substances (EPSs), and biofilm formation. We performed biofilm assays and quantitative real\u2010time PCR to investigate how biofilm volumes and the expression of genes for the synthesis of EPS were affected by SAM supplementation, <jats:italic>agr<\/jats:italic> deletion, or both. We found that exogenously applied SAM induced biofilm formation and that the expression of genes encoding the EPS synthesis machineries was regulated by SAM and\/or Agr QS. Moreover, the gene transcription of components acting in the methyl cycle for SAM synthesis and Agr QS was affected by the signals from the other system. In summary, we reveal an interconnection at the transcriptional level between metabolism and QS in <jats:italic>L.\u00a0monocytogenes<\/jats:italic> and highlight the critical role of metabolite\u2010oriented QS in biofilm development.<\/jats:p>","DOI":"10.1002\/mbo3.1015","type":"journal-article","created":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T13:41:54Z","timestamp":1583415714000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Links between <i>S<\/i>\u2010adenosylmethionine and Agr\u2010based quorum sensing for biofilm development in <i>Listeria monocytogenes<\/i> EGD\u2010e"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2724-2478","authenticated-orcid":false,"given":"Yue\u2010Jia","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Basic Sciences College of Veterinary Medicine Mississippi State University Mississippi State MS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6015-7956","authenticated-orcid":false,"given":"Chinling","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences College of Veterinary Medicine Mississippi State University Mississippi State MS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-66.2.249"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4463-4471.2003"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000442"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05597.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a003566"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1214024109"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.2644-2650.2006"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2004.tb09653.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.83"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1008"},{"issue":"5","key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"e00457\u201000417","DOI":"10.1128\/JB.00457-17","article-title":"CodY\u2010mediated c\u2010di\u2010GMP\u2010dependent inhibition of mammalian cell invasion in Listeria monocytogenes","volume":"200","author":"Elbakush A. M.","year":"2018","journal-title":"Journal of Bacteriology"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms5020015"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2415"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-294"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2011.03.008"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043154"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02891-10"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.4161\/cib.2.4.8610"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8115-8122.2005"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/s130405130"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02045-12"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro821"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-599"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300538"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03715-12"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00259-08"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1188628"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.63.9.3352-3358.1997"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01036-08"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2005.02.015"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005870"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.08.046"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-11-464"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(02)00312-4"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-013-0377-7"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr501114e"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00995-08"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07227-11"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnx207"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07455.x"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0095385"},{"issue":"18","key":"e_1_2_10_43_1","first-page":"9338","article-title":"MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence","volume":"46","author":"Pinheiro J.","year":"2018","journal-title":"Nucleic Acids Research"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2010.05.003"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01492-08"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01359-09"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2354"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06589.x"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00608-07"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08080"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2677"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1004764"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1039\/a804532a"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0124319"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb967"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.57"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/156987"},{"key":"e_1_2_10_58_1","first-page":"13","article-title":"Genome\u2010wide screening of genes required for Listeria monocytogenes biofilm formation","volume":"4","author":"Yong O.","year":"2012","journal-title":"Journal of Biotech Research"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00989"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.5b00812"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-012-0125-4"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmbo3.1015","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.1015","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mbo3.1015","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.1015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T11:24:07Z","timestamp":1693999447000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.1015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,5]]},"references-count":60,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5]]}},"alternative-id":["10.1002\/mbo3.1015"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.1015","archive":["Portico"],"relation":{},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"value":"2045-8827","type":"print"},{"value":"2045-8827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,5]]},"assertion":[{"value":"2019-10-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1015"}}